Figure 5.

The definition of minimum amplicon size (MAS). MAS is the minimum distance (default = 3,000 bp) between the left and right primers when the left and right primer of a pair have consecutive matches of at least eight nucleotides (default value of MPM) with non-target genomic sequences.

Yao et al. BMC Microbiology 2008 8:185   doi:10.1186/1471-2180-8-185
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