Table 4 |
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|
Carotenoid composition of wild-type and transformant X. dendrorhous strains in ppm |
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|
Carotenoid |
UCD 67–385 wt-strain |
T13 (crtRΔBsiWI::hph/crtR+) |
CBS-6938 wt-strain |
CBSTr (crtRΔNdeI::hph) |
|
|
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|
astaxanthin |
102.0 ± (4.8) |
24.0 ± (3.6) |
152.0 ± (8.5) |
ND |
|
phoenicoxanthin |
7.0 ± (0.4) |
8.0 ± (0.4) |
8.0 ± (0.5) |
ND |
|
canthaxanthin |
1.0 ± (0.1) |
3.00 ± (0.06) |
1.0 ± (0.1) |
ND |
|
hydroxy-keto-γ-carotene |
6.0 ± (0.2) |
1.00 ± (0.06) |
ND |
ND |
|
HO-keto-torulene |
ND |
ND |
6.0 ± (1.1) |
ND |
|
keto-γ-carotene |
9.0 ± (0.7) |
11.0 ± (0.7) |
8.0 ± (1.2) |
1.0 ± (0.3) |
|
HO-echinenone |
ND |
ND |
2.0 ± (0.4) |
1.0 ± (0.4) |
|
echinenone |
5.0 ± (0.6) |
16.0 ± (0.5) |
5.0 ± (0.7) |
8.0 ± (1.2) |
|
lycopene |
ND |
1.0 ± (0.01) |
ND |
ND |
|
neurosporene |
ND |
1.00 ± (0.02) |
ND |
ND |
|
γ-carotene |
1.0 ± (0.1) |
2.00 ± (0.05) |
1.0 ± (0.1) |
3.0 ± (0.2) |
|
β-carotene |
9.0 ± (1.7) |
50.0 ± (3.7) |
9.0 ± (1.5) |
114.5 ± (6.6) |
|
phytoene |
0.4 ± (0.3) |
4.2 ± (1.9) |
3.3 ± (1.2) |
1.0 ± (0.4) |
|
NI (25 min)* |
ND |
1.0 ± (0.1) |
ND |
1.0 ± (0.1) |
|
NI (34 min)* |
ND |
2.00 ± (0.04) |
ND |
4.0 ± (0.9) |
|
Total carotenoids |
139.0 ± (3.2) |
122.8 ± (8.0) |
195.3 ± (8.0) |
131.9 ± (7.5) |
|
|
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|
ND: Not detected, NI: Not identified carotenoid. Table values are the average results of three independent experiments, SD in parenthesis. ()*: NI retention time. |
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|
Alcaíno et al. BMC Microbiology 2008 8:169 doi:10.1186/1471-2180-8-169 |
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