|
Primers used for the construction of the ΔflgG and ΔmotAB mutants |
|
| Primer |
Sequence (5' – 3') a |
|
|
|
| KO1flgG |
ATTATGCATGCCGATGAACTATACCTCTCGTCCG |
| KO2flgG |
ATATAGAGCTCGCCAGTAACCGATCGACTGTCACG |
| KO3flgG |
GACGATTAGGTTACATCGGTTTTATCCTCTGTGTC |
| KO4flgG |
GGATAAAACCGATGTAACCTAATCGTCAATCAG |
| KO5flgG |
GCAACTGGCTGCTTTGCGAGC |
| KO6flgG |
GTCCGTGGGTTAACGACACCG |
| KO1motAB |
TATGCATGCGTTACAAATGCTTGAAAGTGAAACAC |
| KO2motAB |
ATATAGAGCTCGTGGCTGATGTTACCAATGATGC |
| KO3motAB |
CGTTACTTTGTCACCTTGGTCGCACGCGATATCCTTTAGC |
| KO4motAB |
GCTAAAGGATATCGCGTGCGACCAAGGTGACAAAGTAACG |
| KO5motAB |
GCAGCTTACTAGGGAATCTCGAGTGG |
| KO6motAB |
CTGCCCGTTGACGTGGCGATCCCG |
|
a restriction sites are underlined | |
Easom and Clarke BMC Microbiology 2008 8:168 doi:10.1186/1471-2180-8-168 |
|