Table 1 |
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Sequences of the oligonucleotide substrates used in this study |
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|
Name |
Sequence |
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|
Oligo 1 (48-mer) |
5'-GGAACAGCTACAGCTGATCATCACCATCACCATCACTAGGATCCGCAT-3' |
|
Oligo 2 (49-mer) |
5'-CACGTGCTAGCCATCACCATCACCATCACGTGAAGACAACAGTCTATCC-3' |
|
Oligo 3 (50-mer) |
5'-CAGGTGCACCAGAACAACCACATCACCATCACCATCACTAGGATCCGCAT-3' |
|
(A)50 (50-mer) |
5'-AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3' |
|
(T)50 (50-mer) |
5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3' |
|
(C)50 (50-mer) |
5'-CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-3' |
|
(G)50 (50-mer) |
5'-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-3' |
|
15 nt (15-mer) |
5'-CCCTGTTAGCACTGT-3' |
|
20 nt (20-mer) |
5'-CCCTGTTAGCACTGTTGTAT-3' |
|
30 nt (30-mer) |
5'-CCCTGTTAGCACTGTTGTATCCAGCATCCT-3' |
|
40 nt (40-mer) |
5'-CCCTGTTAGCACTGTTGTATCCAGCATCCTGTCTTGCAGA-3' |
|
50 nt (50-mer) |
5'-CCCTGTTAGCACTGTTGTATCCAGCATCCTGTCTTGCAGAGCATTCCGAC-3' |
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Sluijter et al. BMC Microbiology 2008 8:167 doi:10.1186/1471-2180-8-167 |
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