Table 3

LAMP primers used

Primer

Sequence

Gene location (bp)

Tlh-FIP

ATG TTT TTA AAT GAA ACG GAG CTC CGG CAA AAA ACG AAG ATG GT (F1c-F2)

392-368 (F1c), 321–339 (F2)

Tlh-BIP

ACG TCG CAA AAC GTT ATC CGG CGA AGA ACG TAA TGT CTG (B1-B2c)

406–425 (B1), 487-467 (B2c)

Tlh-F3

AGC TAC TCG AAA GAT GAT CC (F3)

283–302

Tlh-B3

GGT TGT ATG AGA AGC GAT TG (B3c)

511-492

Tlh-LF

ACC AGT AGC CGT CAA TG (LFc)

367-351

Tlh-LB

TTA GAT TTG GCG AAC GAG A (LB)

445–463

All primers were designed from the sequence of tlh gene of V. parahaemolyticus M36437, submitted to GenBank by Taniguchi et al., 1986 [6].

Yamazaki et al. BMC Microbiology 2008 8:163   doi:10.1186/1471-2180-8-163