Table 2

Sensitivity of the LAMP assay for V. parahaemolyticus
Strain
 Samples
Dilutions of cultures for the assays






10-1
 10-2
 10-3
 10-4
 10-5
V. parahaemolyticus






AQ4980







Pure cultures
 CFU per reaction
 196.3
 19.6
 2.0
 0.2
 0.02


LAMP
 +
 +
 +
 ± (1/3)
 -


PCR
 +
 +
 ± (1/3)
 -
 -









Spiked shrimps
 CFU per reaction
 196.3
 19.6
 2.0
 0.2
 0.02


LAMP
 +
 +
 +
 -
 -


CFU per reaction
 19.6
 2.0
 0.2
 0.02
 0.002


PCR
 +
 -
 -
 -
 ND

+, triplicate assay showed all positive.

±, triplicate assay showed both positive and negative (positive number/tested number).

-, triplicate assay showed all negative.

ND, Not determined.

Yamazaki et al. BMC Microbiology 2008 8:163   doi:10.1186/1471-2180-8-163