Table 6 |
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|
Results of the real-time multiplex PCR assay for the detection of Salmonella from blind culture samples compared to the reported identification from another laboratory. |
||||||
|
Lab. ID |
Real-time multiplex PCR |
Real-time Multiplex PCR Result |
Reported Identification by other lab |
|||
|
aceK |
fliC |
sefA |
sdf |
|||
|
|
||||||
|
BS1 |
+a (23.94) |
-b |
- |
- |
Salmonella |
Gaminara |
|
BS2 |
+ (24.01)c |
- |
+(23.01) |
- |
Gallinarum/Dublin |
Dublin |
|
BS3 |
- |
- |
- |
- |
NDd |
Non-Salmonella |
|
BS4 |
+ (24.16) |
- |
- |
- |
Salmonella |
Branderup |
|
BS5 |
+ (24.77) |
- |
- |
- |
Salmonella |
Manhattan |
|
BS6 |
+ (23.89) |
- |
- |
- |
Salmonella |
Nottingham |
|
BS7 |
- |
- |
- |
- |
ND |
Non-Salmonella |
|
BS8 |
+ (25.36) |
- |
- |
- |
Salmonella |
Anatum |
|
BS9 |
+ (22.93) |
- |
- |
- |
Salmonella |
SanDiego |
|
BS10 |
+ (24.02) |
+ (20.40) |
- |
- |
Typhimurium/Kentucky |
Kentucky |
|
BS11e |
+ (23.10) |
- |
- |
- |
Salmonella |
Non-Salmonella |
|
BS12 |
+ (23.78) |
- |
- |
- |
Salmonella |
London |
|
BS13 |
+ (24.67) |
- |
- |
- |
Salmonella |
Virchow |
|
BS14 |
+ (24.79) |
- |
- |
- |
Salmonella |
Livingstone |
|
BS15 |
- |
- |
- |
- |
ND |
Non-Salmonella |
|
BS16 |
+ (22.90) |
- |
- |
- |
Salmonella |
Derby |
|
BS17 |
- |
- |
- |
- |
ND |
Non-Salmonella |
|
BS18 |
+ (23.42) |
- |
+ (22.03) |
- |
Gallinarum |
Gallinarum |
|
BS19 |
+ (24.33) |
- |
- |
- |
Salmonella |
Saintpaul |
|
BS20 |
+ (25.45) |
- |
- |
- |
Salmonella |
Agona |
|
BS21 |
- |
- |
- |
- |
ND |
Non-Salmonella |
|
BS22 |
+ (23.04) |
+ (20.55) |
- |
- |
Typhimurium/Kentucky |
Typhimurium |
|
BS23 |
+ (22.59) |
+ (20.03) |
- |
- |
Typhimurium/Kentucky |
Typhimurium |
|
BS24 |
+ (23.59) |
- |
- |
- |
Salmonella |
Hadar |
|
BS25 |
+ (23.49) |
- |
- |
- |
Salmonella |
Heidelberg |
|
BS26 |
+ (23.12) |
- |
- |
- |
Salmonella |
Newport |
|
BS27 |
+ (23.73) |
+ (22.68) |
- |
- |
Typhimurium/Kentucky |
Typhimurium |
|
BS28 |
+ (23.96) |
- |
- |
- |
Salmonella |
Reading |
|
BS29 |
+ (22.56) |
+ (21.35) |
- |
- |
Typhimurium/Kentucky |
Typhimurium |
|
BS30 |
+ (22.79) |
- |
- |
- |
Salmonella |
Infantis |
|
|
||||||
|
a + = Salmonella positive by method. b – = Salmonella negative by method. c = CT values in parentheses. d ND = Salmonella not detected. e = BS11 was identified as Salmonella-positive by aceK target and confirmed by biochemical and serology testing, while the another laboratory reported that it was a non-Salmonella isolate. |
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|
O'Regan et al. BMC Microbiology 2008 8:156 doi:10.1186/1471-2180-8-156 |
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