Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes

Additional file 1:

Host range analysis. Propionibacterium acnes isolates were plated on TSBA as an overlay assay. Phages isolated from these strains were added to each P. acnes isolate and incubated for two days at anaerobic conditions and were analyzed for plaques. A plus (+) indicates that the bacterial isolate was lysed, and the number of + indicates the quality and extent of the lysis in the bacteria. A minus (-) indicates that no plaques were observed. P. acnes strain KPA171202 is the recently sequenced P. acnes strain. Bacterial isolates AD2-AS52 have inducible phages while bacterial isolates AD1-KPA171202 do not have inducible phages.

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Additional file 2:

Phylogenetic tree of P. acnes phages based on partial sequencing on a gene encoding a putative major head protein. A part of the gene encoding the putative structural protein major head protein was amplified and sequenced. Obtained nucleotide sequences were compared using MacVector ClustalW Alignment and a phylogenetic tree was constructed using UPGMA and uncorrected p-values with 1000 replications for bootstrap. The phages were divided into two distinct groups, with the recently sequenced phage PA6 forming a third group.

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