Table 1

Bacterial strains and plasmids used in this study
Strains or Plasmids	Characteristics	Reference or source

Strains
Vibrio scophthalmi A089	Turbot isolate, CECT 4638T	[18]
Vibrio scophthalmi A102	Turbot isolate	[18]
Vibrio scophthalmi A089-aiiA strain	A089 strain with the aiiA gene cloned into pACYC184 plasmid, Cl^r	This study
Vibrio scophthalmi A102-aiiA strain	A102 strain with the aiiA gene cloned into pACYC184 plasmid, Cl^r	This study
E. coli DH5α	F^-Φ80d lacZΔM15endA1hsdR17(r_k^-m_k^-) supE44 thi-1 gyrA96Δ(lacZYA-argF)	[37]
E. coli HB101 (pRK2073)	Mobilizing strain; Sp^r	Biomedal
E. coli One Shot cells	Used for transformation Experiments	Invitrogen
Sensor strains
A. tumefaciens NTL4(pZLR4)	AHLs sensor strain, Gm^r	[34]
C. violaceum CV026	AHLs sensor strain	[25]
V. harveyi BB866	AI-1 indicator strain, BB120 luxP::Tn5	[2]
V. harveyi BB170	AI-2 indicator strain, BB120 luxN::Tn5	[42]
V. harveyi BB120	wild-type strain	[2]
P. putida F117 (pAS-C8)	AHLs sensor strain, Gm^r	[27]
P. putida F117 (pKR-C12)	AHLs sensor strain, Gm^r	[27]
Plasmids
pCR2.1-TOPO	TA cloning vector; Amp^r, Km^r	Invitrogen
pGEM-T-easy	TA cloning vector; Amp^r	Promega
pACYC184	Broad-host range expression vector, Tc^r, Cl^r	[36]
pACYC184-aiiA	Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Cl^r	This study
pME6863	Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Tc^r	[4]

Antibiotic abbreviations used in this study: Ampicillin (Amp), Kanamycin (Km), Spectinomycin (Sp), Chloramfenicol (Cm), Trimethoprim (Tri), Tetracycline (Tc); Spanish Type Culture Collection (CECT).
García-Aljaro et al. BMC Microbiology 2008 8:138 doi:10.1186/1471-2180-8-138