Table 1

Bacterial strains and plasmids used in this study

Strains or Plasmids

Characteristics

Reference or source


Strains

Vibrio scophthalmi A089

Turbot isolate, CECT 4638T

[18]

Vibrio scophthalmi A102

Turbot isolate

[18]

Vibrio scophthalmi A089-aiiA strain

A089 strain with the aiiA gene cloned into pACYC184 plasmid, Clr

This study

Vibrio scophthalmi A102-aiiA strain

A102 strain with the aiiA gene cloned into pACYC184 plasmid, Clr

This study

E. coli DH5α

F-Φ80d lacZΔM15endA1hsdR17(rk- mk-) supE44 thi-1 gyrA96Δ(lacZYA-argF)

[37]

E. coli HB101 (pRK2073)

Mobilizing strain; Spr

Biomedal

E. coli One Shot cells

Used for transformation Experiments

Invitrogen

Sensor strains

A. tumefaciens NTL4(pZLR4)

AHLs sensor strain, Gmr

[34]

C. violaceum CV026

AHLs sensor strain

[25]

V. harveyi BB866

AI-1 indicator strain, BB120 luxP::Tn5

[2]

V. harveyi BB170

AI-2 indicator strain, BB120 luxN::Tn5

[42]

V. harveyi BB120

wild-type strain

[2]

P. putida F117 (pAS-C8)

AHLs sensor strain, Gmr

[27]

P. putida F117 (pKR-C12)

AHLs sensor strain, Gmr

[27]

Plasmids

pCR2.1-TOPO

TA cloning vector; Ampr, Kmr

Invitrogen

pGEM-T-easy

TA cloning vector; Ampr

Promega

pACYC184

Broad-host range expression vector, Tcr, Clr

[36]

pACYC184-aiiA

Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Clr

This study

pME6863

Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Tcr

[4]


Antibiotic abbreviations used in this study: Ampicillin (Amp), Kanamycin (Km), Spectinomycin (Sp), Chloramfenicol (Cm), Trimethoprim (Tri), Tetracycline (Tc); Spanish Type Culture Collection (CECT).

García-Aljaro et al. BMC Microbiology 2008 8:138   doi:10.1186/1471-2180-8-138

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