Detection of AHLs by thin layer chromatography (TLC) with the A. tumefaciens NTL4 (pZLR4) strain used as a biosensor (A) and AHL detection along the growth curve in mTSB medium (B). A) lane 1: C8-HSL standard (Fluka); lane 2: Vibrio scophthalmi A089 strain; lane 3: V. scophthalmi A102 strain; lane 4: Negative control, mTSB alone; lane 5: Negative control, no sample added; 6: C6-HSL standard (Fluka). B) Arrow indicates the beginning of the detection of the three signalling spots, which were detected at the same time from the mid-log phase.
García-Aljaro et al. BMC Microbiology 2008 8:138 doi:10.1186/1471-2180-8-138