Figure 4.

Effect of modifications of the Spx CXXC motif on PQ induced expression of msrA. RT-PCR analysis of msrA expression in spx mutant complemented with wild-type spx (BSY5051) or spx10A13A allele (BSY5052) was performed. 100% expression for each strain is defined as the expression of msrA without any oxidant treatment. Strains were grown in ED minimal medium to mid-exponential phase and incubated with or without PQ (100 μM) for 15 min.