Table 2

Primers for qPCR

Gene

Forward primer

Reverse primer

dnaE

5'-AATCACACTCTGCCGCTTATG-3'

5'-CCATCCGCCAGCACTCAT-3'

polA

5'-GCTGGCTTGCGGATGTAGAT-3'

5'-AGCACGGCGGTCACTTCA-3'

gapA

5'-CCATCCGTGTTACCGCAGAG-3'

5'-TCTTAGCACCAGCAGCAATGT-3'

fleC

5'-GTAGTAGCGGTTTTCAGTGCTGT-3

5'-CCGGTGGTATTTCAGGTGAT-3'

hutI

5'-ACTCGCAGGTGTCACTTG-3'

5'-GAGCCAGTAAGCCAATTCAG-3'

hutU

5'-CGATATTCTTCCCAGCTCCA-3'

5'-GCAACAGATTTTGATGATGCG-3'

carA

5'-AAGGCAGTTGGACTCTGGAA-3'

5'-CCGTTGGATAAGAAAATGCC-3'

carB

5'-GTTAATTGGCGGCATTATGG-3'

5'-GACTTCAATCAGGTAAACTTC-3'

pyrB

5'-CCGATTGTCGTTTGAAACCT-3'

5'-ATTGACCACCGGCACATTGCC-3'

deoB

5'-GCTGACTGAAGGCGGTTACAAT-3'

5'-GGTGCTGGCGGTTCAACTG-3'

acrE

5'-AGATTCTGGCTAACGACTACG-3'

5'-ATCACCTGAGCGATGTATGG-3'

oppA1

5'-TCAAGAACAGCCAGAAGCCCTA-3'

5'-AAGCGAGCGTGGTTGGAATAC-3'

oppB1

5'-TGGCGGTGATAGTGGATT-3'

5'-TGATCTCGGCAATAACTTCC-3'

oppC1

5'-ACCAAGATCCGTATGTCATT-3'

5'-TACCGAGCCAGTTAGTGT-3'

oppDF

5'-TGAGATTATCCAGTGCCTTGA-3'

5'-TAGTCAGAACCGCCATAGC-3'

oppB2

5'-GCATTCCTACTGGCGGTGATT-3'

5'-AGGACTAACAGAGGAGCAACGA-3'

dppB1

5'-CCTTACTGACAGTGATTGC-3'

5'-ATAATGGCGGTGGTATCG-3'

dppB2

5'-ACCATTCCTCTCGCCGTTAT-3'

5'-GACCACAATCACTCGCATCC-3'

Column 1 presents the genes that were analyzed.

Columns 2 and 3 indicate the primers that were used for the qPCR reaction. Sequence information was obtained from Sanger [22].

Townsend et al. BMC Microbiology 2008 8:12   doi:10.1186/1471-2180-8-12