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Primers for qPCR |
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| Gene |
Forward primer |
Reverse primer |
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| dnaE |
5'-AATCACACTCTGCCGCTTATG-3' |
5'-CCATCCGCCAGCACTCAT-3' |
| polA |
5'-GCTGGCTTGCGGATGTAGAT-3' |
5'-AGCACGGCGGTCACTTCA-3' |
| gapA |
5'-CCATCCGTGTTACCGCAGAG-3' |
5'-TCTTAGCACCAGCAGCAATGT-3' |
| fleC |
5'-GTAGTAGCGGTTTTCAGTGCTGT-3 |
5'-CCGGTGGTATTTCAGGTGAT-3' |
| hutI |
5'-ACTCGCAGGTGTCACTTG-3' |
5'-GAGCCAGTAAGCCAATTCAG-3' |
| hutU |
5'-CGATATTCTTCCCAGCTCCA-3' |
5'-GCAACAGATTTTGATGATGCG-3' |
| carA |
5'-AAGGCAGTTGGACTCTGGAA-3' |
5'-CCGTTGGATAAGAAAATGCC-3' |
| carB |
5'-GTTAATTGGCGGCATTATGG-3' |
5'-GACTTCAATCAGGTAAACTTC-3' |
| pyrB |
5'-CCGATTGTCGTTTGAAACCT-3' |
5'-ATTGACCACCGGCACATTGCC-3' |
| deoB |
5'-GCTGACTGAAGGCGGTTACAAT-3' |
5'-GGTGCTGGCGGTTCAACTG-3' |
| acrE |
5'-AGATTCTGGCTAACGACTACG-3' |
5'-ATCACCTGAGCGATGTATGG-3' |
| oppA1 |
5'-TCAAGAACAGCCAGAAGCCCTA-3' |
5'-AAGCGAGCGTGGTTGGAATAC-3' |
| oppB1 |
5'-TGGCGGTGATAGTGGATT-3' |
5'-TGATCTCGGCAATAACTTCC-3' |
| oppC1 |
5'-ACCAAGATCCGTATGTCATT-3' |
5'-TACCGAGCCAGTTAGTGT-3' |
| oppDF |
5'-TGAGATTATCCAGTGCCTTGA-3' |
5'-TAGTCAGAACCGCCATAGC-3' |
| oppB2 |
5'-GCATTCCTACTGGCGGTGATT-3' |
5'-AGGACTAACAGAGGAGCAACGA-3' |
| dppB1 |
5'-CCTTACTGACAGTGATTGC-3' |
5'-ATAATGGCGGTGGTATCG-3' |
| dppB2 |
5'-ACCATTCCTCTCGCCGTTAT-3' |
5'-GACCACAATCACTCGCATCC-3' |
|
Column 1 presents the genes that were analyzed. Columns 2 and 3 indicate the primers that were used for the qPCR reaction. Sequence information was obtained from Sanger [22]. | ||
Townsend et al. BMC Microbiology 2008 8:12 doi:10.1186/1471-2180-8-12 |
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