Table 2 |
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|
Primers used in this study |
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|
Primer Designation |
Primer sequence 5' – 3' |
Ta (°C) |
Product size (bp) |
|
|
|||
|
VPaI-2 |
|||
|
|
|||
|
VP0634F |
GGGGGAAATAAATGTCTGAAGG |
52.1 |
1363 |
|
VP0634R |
AACACGCCAAGACTCTC |
||
|
VP0637F |
GGAATAACTCAGAGCTTCG |
52.8 |
1848 |
|
VP0640R |
TAGGCAGTCGTAATTCG |
||
|
VP0643F |
ATACGCCTGATTGCTTC |
52.0 |
1558 |
|
VP0643R |
TGGTACTATCAACGCCG |
||
|
VP0644F |
CGTGCTTTTTCTCTTGC |
51.1 |
970 |
|
VP0644R |
CCATATTGCTAGTTAGCTCG |
||
|
|
|||
|
VPaI-3 |
|||
|
|
|||
|
VP1069F |
TAGGGTCGGTGGTGTACTTG |
52.8 |
2041 |
|
VP1069R |
GACTCCACTATTGGTTTA GC |
||
|
VP1072F |
AGAGTCAGAGGAAAGGGAGG |
50.3 |
2275 |
|
VP1073R |
GTAAATGTTGTGGGTGC |
||
|
VP1079F |
CTGTCTTCATGCCTTTG |
51.0 |
1691 |
|
VP1079R |
CGCCATTGCTAAACGTC |
||
|
VP1083F |
CTTACTTATTGGAGGCTGG |
52.1 |
2127 |
|
VP1083R |
GGTGGGTATAAAGGTAACG |
||
|
VP1095F |
TCTGGTTCGGTATTTGG |
52.7 |
1166 |
|
VP1096R |
CGCAGCATTTCTTGAAG |
||
|
|
|||
|
VPaI-7 |
|||
|
|
|||
|
VPA1308F |
TTAGAACGCATGAGCACCG |
53.1 |
1844 |
|
VPA1309R |
CCACCAAAGTGTTTGTGAG |
||
|
VPA1312F |
CTACTATCATCACGACGTG |
49.4 |
1487 |
|
VPA1314R |
CGTGCTTATAGCCAGAC |
||
|
VPA1317F |
GACAGACAGAGATACGCTG |
50.9 |
1366 |
|
VPA1320R |
TTCAGAGGTGTCGCACTTCG |
||
|
VPA1321F |
CGTGGTGGTTAGTGAATC |
49.1 |
886 |
|
VPA1321R |
AGAGTTGGTTTCGCAGG |
||
|
VPA1321F |
GACCACTATATTGTTCTCCG |
49.4 |
1480 |
|
VPA1323R |
CTCAGGGATAAATAGGGATG |
||
|
VPA1331F |
CCAATAATCACCCTCCG |
49.9 |
1857 |
|
VPA1334R |
CTCAGGGATAAATAGGGATG |
||
|
VPA1340F |
GTCCTTGATTACACCATTGG |
51.8 |
1618 |
|
VPA1343R |
GCACGTAACATCTAAGTTCGTG |
||
|
VPA1350F |
TGCATCGTCATTTCTCC |
50.4 |
2630 |
|
VPA1354R |
CGTAGATTTCATGGCAG |
||
|
VPA1363F |
TTTCACTAATGCTGCGG |
51.1 |
2308 |
|
VPA1365R |
GGTCAATATGGCACTATGC |
||
|
VPA1380F |
TTAGGGGTGTTATGCCG |
48.5 |
816 |
|
VPA1380R |
TTACTGTCTCTGTTGCAGG |
||
|
VPA1390F |
CCACAACACAAACTGTCC |
50.9 |
2606 |
|
VPA1393R |
AATCCAAGGGGAGTGAC |
||
|
VPA1394F |
AACGCCGAATTAACCGC |
53.2 |
2356 |
|
VPA1395R |
TCACCCCAATGTACCGTCTG |
||
|
VPA1397F |
GCGGAGCTGTAATGAAATG |
52.9 |
793 |
|
VPA1398R |
CAACCAACGTATTGTAGCAG |
||
|
VPA1400F |
ATAGGTCTGTGTAACCCG |
52.3 |
1964 |
|
VPA1401R |
GGTAAAGCTGCGATGAC |
||
|
VP0085F |
TGCTCGCTGCTATCTAC |
53.0 |
1160 |
|
VP0085R |
CGTTAAATACGCCAGTTGC |
||
|
VP0220F |
CCCTCAAGTGATTGATCC |
53.0 |
1878 |
|
VP0220R |
AAGATAGCCCCTTGTGG |
||
|
VP1399F |
CATCTCTTGCTCTTGGAG |
51.0 |
1312 |
|
VP1400R |
TGAGGTCTACAATGAGTCAG |
||
|
VP1415F |
CGCAATTAAAGGCAGTACG |
51.0 |
2274 |
|
VP1416R |
GACTGAATAAGAGTGCTCG |
||
|
VP1556F |
TCCCGATTGTAAGTTGC |
53.0 |
1957 |
|
VP1558R |
AGCTAATGCGAATGAGC |
||
|
VPA0446F |
ACGTTCTTTTGGGATGG |
53.0 |
2376 |
|
VPA0447R |
ACCGAAGCCTTAACACG |
||
|
VPA0450F |
AATGCGAAAGAAGGCGATAC |
60.0 |
1337 |
|
VPA0450R |
TGCGCTTGTAGATGAGTTGG |
||
|
VPA0891F |
GTCGCTCTTTATGTTGC |
50.5 |
615 |
|
VPA0891R |
GAAGCCTGTATCAACTGTC |
||
|
VPA0894F |
TAATGGTCGATGCACTG |
54.0 |
1215 |
|
VPA0894R |
GGATGAGCAAGTCAGTAGC |
||
|
VPA0952F |
CGAGTGATCCAGTTTTACAC |
52.0 |
2720 |
|
VPA0953R |
AACTACCAGCTAGAAGTGG |
||
|
VPA0992F |
CCGACATAAAGGGATACTC |
52.0 |
1926 |
|
VPA0992R |
GAAGAAGCACTTGCTCTC |
||
|
VPA1443F |
CAATCAGCAGCCAGTCGTTA |
60.0 |
1233 |
|
VPA1443R |
CCGGATGTCAAACGGTACTT |
||
|
VPA1503F |
GTTCGACAATGGCATGTGAG |
60.0 |
801 |
|
VPA1503R |
CGCCAGTATCGACATCACTC |
||
|
VPA1655F |
CCGTTTTGCTGATGCTACTG |
60.0 |
816 |
|
VPA1655R |
ATGACCACAGTTCCGGAGAG |
||
|
VPGyrBF |
GTA CTG AAG GGT CTG GAT GC |
54.6 |
742 |
|
VPGyrBR |
ACT GCA TTG CCA CTT CTA CC |
||
|
VPmdhF |
TGAAAGTAGCCGTTATTGG |
54.0 |
901 |
|
VPmdhR |
CCATTTAGCGTTTCTAGCATTC |
||
|
VPGroEL1F |
TTTCGGTGCTCCAACCATC |
55.4 |
737 |
|
VPGroEL1R |
GCATTGCTTTACGACGGTC |
||
|
|
|||
|
Boyd et al. BMC Microbiology 2008 8:110 doi:10.1186/1471-2180-8-110 |
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