Figure 4.

Correlation of microaray and real-time PCR. Real-time PCR measurements of the mRNA levels for seven representative genes whose expression were affected in the array. Upper panel shows a direct comparison of the changes in a log2 scale for PAO1 versus control and the lower panel shows the same genes for PA14 versus control. Blue bars corresponded to quantitative real time PCR and the purple bars to the array data. The array data and the real time PCR displayed are the combination of three independent biological experiments. The correlation coefficients were: R² = 0.87 for PAO1 and R² = 0.91 for PA14.