Figure 1.

PA14 is more virulent than PAO1 in SM-plating assay. Approximately 100 D. discoideum cells were cultivated in SM-plates with the indicated proportion of Klebsiella and Pseudomonas strains (PAO1 or PA14) previously grown and adjusted to the same optical density. Plates were maintained at 22°C for 5 days. Growth of D. discoideum is severely affected by the presence of Pseudomonas but the inhibition is stronger when PA14 is used.