Figure 1.

Detection of Hsd polypeptides in total cell extracts using the anti-EcoAI antibody. lane 1, purified EcoAI enzyme – standard; lane 2, extract from E. coli DH10B [pFFP30] expressing the EcoAI system; lanes 3, 4, extracts from E. coli DH10B [BAC-C4/1] expressing the EcoAO83I; lane 5,E. coli A0 34/86 (O83: K24: H31), EcoAO83I expressed from the chromosome; lane 6,E. coli DH10B [BAC] – control with no R-M system; The position of the Hsd subunits is indicated, molecular weights in kD are in bracket.