Figure 4.

DENV-2 antigen in the tracheal system of Chetumal mosquitoes following infection. DENV-2 antigen was detected by IFA in trachea of Chetumal mosquitoes at 2, 3, 4, 5, and 7 dpi (n= 40–50). Midgut- associated tracheal system (A) and uninfected trachea using light (B) and fluorescence (C) microscopy. Green arrows point to portions of DENV-2 infected trachea (E-L) (200×).