Table 3

Influence of mismatch type on weighted mismatch calculation

Probe

Target

Probe

Target

Probe

Target

Probe

Target


A -

A

1.0

G -

A

1.0

C -

A

0.7

T -

C

1.0

-

C

0.4

-

G

1.0

-

C

1.0

-

G

1.0

-

G

1.2

-

T

1.0

-

T

1.0

-

T

1.0


Weights of mismatches due to the type of mismatched bases. A mismatch of adenine on the probe with cytosine on the target sequence is weighted with 0.4, whereas a mismatch of the same probe with a guanine in the target sequence is weighted with 1.2.

Wiesinger-Mayr et al. BMC Microbiology 2007 7:78   doi:10.1186/1471-2180-7-78

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