Figure 1.

Normalised signal intensities of all hybridisation experiments listed by probe and species. The raw signal values were first normalised using quantile normalisation, and then averaged across spot-replicates and hybridisation-replicates (real values were divided by 1000 for better visualisation). No cut off value or signal limit was set in order to use absolute intensities for normalisation and table calculation. Background signals were subtracted prior to statistical evaluation. Background corrected hybridisation signals of 5001 – 10000, 10001 – 20000, and > 20001, are indicated in grey, dark grey and black, respectively. Normalised values lower than 5000 are not colour-coded. For calculations absolute values were used without defining a threshold that led to indication of low signals even when signals were flagged negative by the GenePix analysis software. Species are listed according to the phylogenetic relation of 16S and 18S rRNA sequences. Probes are sorted by species specificity. Abbreviations of probe names are listed in table 1.

Wiesinger-Mayr et al. BMC Microbiology 2007 7:78   doi:10.1186/1471-2180-7-78
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