Email updates

Keep up to date with the latest news and content from BMC Microbiology and BioMed Central.

Open Access Highly Accessed Research article

Microarray for serotyping of Bartonella species

Cyrille J Bonhomme, Claude Nappez and Didier Raoult*

Author affiliations

Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine de Marseille, 13385 Marseille cedex 05, France

For all author emails, please log on.

Citation and License

BMC Microbiology 2007, 7:59  doi:10.1186/1471-2180-7-59

Published: 25 June 2007

Abstract

Background

Bacteria of the genus Bartonella are responsible for a large variety of human and animal diseases. Serological typing of Bartonella is a method that can be used for differentiation and identification of Bartonella subspecies.

Results

We have developed a novel multiple antigenic microarray to serotype Bartonella strains and to select poly and monoclonal antibodies. It was validated using mouse polyclonal antibodies against 29 Bartonella strains. We then tested the microarray for serotyping of Bartonella strains and defining the profile of monoclonal antibodies.

Bartonella strains gave a strong positive signal and all were correctly identified. Screening of monoclonal antibodies towards the Gro EL protein of B. clarridgeiae identified 3 groups of antibodies, which were observed with variable affinities against Bartonella strains.

Conclusion

We demonstrated that microarray of spotted bacteria can be a practical tool for serotyping of unidentified strains or species (and also for affinity determination) by polyclonal and monoclonal antibodies. This could be used in research and for identification of bacterial strains.