Table 1 |
||
|
Oligonucleotides used in this study |
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|
Letter |
Sequence (5'-3') |
Target |
|
|
||
|
Blot hybridization and primer extension |
||
|
A |
TTTTTTTGTGCGTGTG |
MINA SL |
|
B |
GCCTGCTCGCCGAGGTGTGG |
" |
|
C* |
CGCGTGCTTGTGCTGGTGTGCG |
" |
|
D |
CAGCGGGGCTGCTGTGTGGT |
" |
|
E |
GCCCGGACATGATGGGCGGTGTGTG |
" |
|
F |
GTTCCGGAAGTTTCGCATAC |
" |
|
G |
TCTCTCTCTCTCTCACCATC |
pX-tSL |
|
H |
CATCATCAACTGTCTCTTGT |
" |
|
I |
TTTCAAGGCTTCCCGAACGC |
" |
|
J |
GCGTGTGTTGAGCCGTCCACCGTA |
" |
|
K |
ACTTCCTCGAGGCTGAA |
" |
|
L |
ATCCTCTAGAGCGGCCGCCA |
" |
|
Revere complement probes |
||
|
C'* |
CGCACACCAGCACAAGCACAAGCACGCG |
MINA SL |
|
E' |
CACACACCGCCCATCATGTCCGGGC |
" |
|
F' |
GTATGCGAAACTTCCGGAAC |
" |
|
G' |
GATGGTGAGAGAGAGAGAGA |
pX-tSL |
|
H' |
ACAAGAGACAGTTGATGATG |
" |
|
K' |
TTCAGCCTCGAGGAAGT |
" |
|
FISH |
||
|
U3 |
CCTCCTGCCGTCAATCGAAACGCCT |
U3 snoRNA |
|
RRNA |
AGCCAGCAGGCACGCCAGTGAGGCATGTCTGG |
rDNA IGS |
|
NSTAG |
TCCGGAAGTTTCGCATACTTCCTCGAGGCTGAA |
pX-tSL |
|
|
||
|
*: denotes oligonucleotides used for primer extension mapping. Underlined bases represent Tamara fluorophore labels. |
||
|
Hitchcock et al. BMC Microbiology 2007 7:44 doi:10.1186/1471-2180-7-44 |
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