Figure 6.

Binding of the PreIC to the -60 PSE inducesbending of the DNA. A. Location of the -60 PSE (open box) relative to the 409-bp restriction fragments released from pCY7. B = BamHI; H = HindIII; RI = EcoRI; RV = EcoRV. B. Diagram showing the formula used to calculate the bending angle from measured parameters [41,57]. L1 = length of DNA; L2 = end-to-end distance of bent EcoRV-digested DNA. M_x = M2/M1 (Fig 1C) ≅ L2/L1 (Fig 1B). C. EMSA analysis of different pCY7 restriction fragments incubated with 30% ammonium sulphate cut of L. tarentolae nuclear extract. M1 = mobility of EcoRI digested DNA; M2 = mobility of EcoRV digested DNA. W = wild type binding site; M = 10-bp transversion mutation of the binding site.