Figure 1.

Western blots of 10% slab gels loaded with Hwp1 antigens of C. albicans stained with silver stain (panel A), an anti-Myc monoclonal antibody (panel B, lane 1), an anti-HSV monoclonal antibody (panel B, lanes 2 and 3), a polyclonal antiserum against the purified recombinant N-Hwp1 fragment (panel C) and human sera (panel D). Gels in panels A-C were loaded with rHwp1N13KV-c~myc (lane 1), N-Hwp1 (lane 2) and int-Hwp (lane 3). Panel D shows the reactivity of N-Hwp1 with sera from two patients with invasive candidiasis (lanes 1 and 2) and from two patients without invasive candidiasis (lanes 3 and 4). Molecular masses (in kDa) of standard proteins are listed to the left of the gels.