Table 1

Regulatory dependence of type III secreted effectors under SPI-1 inducing conditions.


A
 B
 C
 D
 E
 F
 G
 H
 I


wild type
 MM+ pWSK29
 MM sprB+
 MM hilC+
 MM hilD+
 MM hilA +
 MM invF+ sicA +
 MM ssrB+
 MM sirA +
1
 sopA-lacZ
 7685 ± 1026
 23 ± 1
 1 ± 2
 0 ± 2
 1 ± 2
 9 ± 3
 4982 ± 625
 9 ± 3
 1 ± 1
2
 sopB-lacZ
 10981 ± 922
 148 ± 11
 85 ± 2
 75 ± 8
 82 ± 4
 199 ± 15
 56160 ± 4842
 3095 ± 1711
 113 ± 8
3
 sopE2-lacZ
 14251 ± 4224
 1872 ± 252
 2425 ± 748
 2477 ± 281
 1093 ± 391
 3735 ± 65
 31415 ± 1109
 3963 ± 90
 2574 ± 69
4
 slrP-lacZ
 385 ± 44
 305 ± 5
 3511 ± 349
 295 ± 164
 448 ± 78
 403 ± 87
 1282 ± 138
 308 ± 39
 621 ± 23
5
 sspHl-lacZ
 796 ± 196
 689 ± 26
 697 ± 165
 668 ± 98
 949 ± 103
 960 ± 146
 2906 ± 585
 827 ± 165
 666 ± 67
6
 sspH2-lacZ
 360 ± 34
 326 ± 38
 412 ± 128
 225 ± 16
 389 ± 71
 453 ± 90
 359 ± 24
 15685 ± 1481
 296 ± 11
7
 sifA-lacZ
 21664 ± 538
 10321 ± 719
 9970 ± 528
 9232 ± 665
 9611 ± 887
 10176 ± 817
 10329 ± 1153
 14085 ± 429
 16738 ± 1612
8
 sifB-lacZ
 65 ± 2
 939 ± 81
 1393 ± 849
 19 ± 5
 1537 ± 470
 809 ± 150
 30 ± 6
 14334 ± 2904
 25 ± 6
9
 sseI-lacZ
 70 ± 4
 60 ± 10
 69 ± 22
 32 ± 2
 43 ± 5
 61 ± 15
 47 ± 10
 5154 ± 1291
 60 ± 15
10
 sseG-lacZ
 1931 ± 36
 2046 ± 440
 1153 ± 257
 3001 ± 297
 2083 ± 442
 2872 ± 314
 2444 ± 554
 25454 ± 314
 919 ± 73

The lac fusion being measured is listed in the left column. The strain background is listed along the top. Each strain harbors a lac fusion to an effector and a plasmid encoding a regulator or the vector control. The values in bold are those showing greater than 3-fold differences compared to the MM + pWSK29 strain that are discussed in the text. Each value represents the mean of at least three experiments ± standard deviation. MM, multi-regulator mutant [Δ(avrA-invH)1 ssrB::cat sirA4::hyg].

Dieye et al. BMC Microbiology 2007 7:3   doi:10.1186/1471-2180-7-3