|
SnaBI-SpeI PFGE classification of Map strains. |
||||||||
| Country |
PFGE profile |
Locations |
Host sp |
No. of isolates (%) |
No. of herds or flocks (%) |
Other types in same herd/flock (No. of herds/flocks) |
Primary Isolation |
IS1311 type |
|
|
||||||||
| Spain |
1-1 |
BC, As, Ar, Na, Cat |
Cattle |
75 (32.33) |
40 (30.53) |
1–20 (1), 2-1 (6), 22-1 (1), 23-16 (1), 28–30 (1), 31-1 (1) |
HEY, LJ, 7H11* |
C type |
| *1–10 |
BC |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *1–20 |
Na |
Cattle |
1 (0.43) |
1 (0.76) |
1-1 (1), 31-1 (1) |
HEY |
C type |
|
| *1–27 |
BC |
Cattle |
3 (1.29) |
1 (0.76) |
HEY |
C type |
||
| 2-1 |
BC, As, Can, Ga, CL, Ar, Na, Cat, CM |
Cattle |
121 (52.16) |
76 (58.02) |
1-1 (6), 2–10 (2), 2–15 (1), 2–31 (1), 22-1 (1), 23-16 (2), 28–30 (1) |
HEY, LJ, 7H11* |
C type |
|
| 2–5 |
As |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| 2–10 |
Can, CL, Ex |
Cattle |
3 (1.29) |
3 (2.29) |
2-1 (2) |
HEY |
C type |
|
| *2–12 |
BC |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *2–14 |
Ga |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *2–15 |
BC, Ma |
Cattle |
3 (1.29) |
3 (2.29) |
2-1 (1), 23-16 (1) |
HEY |
C type |
|
| *2–25 |
CL |
Cattle |
2 (0.86) |
1 (0.76) |
HEY |
C type |
||
| *2–31 |
CL |
Cattle |
1 (0.43) |
1 (0.76) |
2-1 (1) |
HEY |
C type |
|
| *20–27 |
BC |
Cattle |
2 (0.86) |
2 (2.29) |
HEY |
C type |
||
| *21-1 |
Can |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *22-1 |
Ar |
Cattle |
1 (0.43) |
1 (0.76) |
1-1 (1), 2-1 (1) |
HEY |
C type |
|
| *23-16 |
BC |
Cattle |
7 (3.02) |
7 (5.34) |
1-1 (1), 2-1 (2), 2–15 (1) |
HEY |
C type |
|
| *24-19 |
Ma |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *27-26 |
BC |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *28–30 |
BC |
Cattle |
1 (0.43) |
1 (0.76) |
1-1 (1), 2-1 (1) |
HEY |
C type |
|
| *31-1 |
Na |
Cattle |
1 (0.43) |
1 (0.76) |
1-1 (1), 1–20 (1) |
HEY |
C type |
|
| *32-1 |
BC, Na |
Cattle |
2 (0.86) |
2 (1.53) |
HEY |
C type |
||
| *33-1 |
BC |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| *40-1 |
CL |
Cattle |
1 (0.43) |
1 (0.76) |
HEY |
C type |
||
| 2-1 |
Ar |
Sheep |
1 (5.88) |
1 (10.0) |
LJ |
C type |
||
| *25-23 |
Ar |
Sheep |
1 (5.88) |
1 (10.0) |
LJ |
S type |
||
| *26-24 |
BC, Na |
Sheep |
3 (17.65) |
2 (20.0) |
LJ, 7H9*+7H11 |
S type |
||
| *30-11 |
BC |
Sheep |
2 (11.76) |
2 (20.0) |
39-17 (2), 39-21 (1), 41-32 (1) |
7H11 |
S type |
|
| *35-29 |
BC |
Sheep |
1 (5.88) |
1 (10.0) |
7H11*, 7H9+7H11 |
S type |
||
| *36-18 |
BC |
Sheep |
1 (5.88) |
1 (10.0) |
38-22 (1), 39-17 (1) |
7H11 |
S type |
|
| *38-22 |
BC |
Sheep |
1 (5.88) |
1 (10.0) |
36-18 (1), 39-17 (1) |
7H11 |
S type |
|
| *39-17 |
BC, Na |
Sheep |
4 (23.53) |
4 (40.0) |
30-11 (2), 36-18 (1), 38-22 (1), 39-21 (1), 41-32 (1) |
7H11 |
S type |
|
| *39-21 |
BC |
Sheep |
2 (11.76) |
2 (20.0) |
30-11 (1), 39-17 (1) |
7H11 |
S type |
|
| *41-32 |
BC |
Sheep |
1 (5.88) |
1 (10.0) |
30-11 (1), 39-17 (1) |
7H11 |
S type |
|
| 2-1 |
BC |
Goat |
2 (28.57) |
1 (20.0) |
HEY, 7H11* |
C type |
||
| 15-1 |
An |
Goat |
1 (14.29) |
1 (20.0) |
34-28 (1) |
7H11*, 7H9+7H11 |
C type |
|
| 16-11 |
CL, An, IB |
Goat |
3 (42.86) |
3 (60.0) |
LJ, 7H9+7H11 |
S type |
||
| *34-28 |
An |
Goat |
1 (14.29) |
1 (20.0) |
15-1 (1) |
7H9+7H11 |
S type |
|
| *37-1 |
CM |
Deer |
2 (100.0) |
1 (100.0) |
HEY |
C type |
||
| 2-1 |
CM |
Wild Boar |
1 (100.0) |
1 (100.0) |
HEY, 7H11* |
C type |
||
|
|
||||||||
| India |
*29-1 |
Mathura |
Sheep |
2 (100.0) |
1 (100.0) |
HEY |
B type |
|
| *29-1 |
Farah |
Goat |
5 (100.0) |
1 (100.0) |
HEY |
B type |
||
|
|
||||||||
| USA |
2-1 |
Montana |
Bison |
3 (100.0) |
1 (100.0) |
LJ |
B type |
|
|
Details of SnaBI-SpeI multiplex profiles of the 269 Map isolates studied by PFGE: geographic origin, number of isolates, number of herds/flocks of the indicated host species, primary isolation media and IS1311 PCR-REA type. Profiles in boldface correspond to pigmented isolates. *novel profiles found in this study. Abbreviations: An = Andalucía; Ar = Aragón; As = Asturias; BC = Basque Country; Can = Cantabria; Cat = Cataluña; CL = Castilla y León; CM = Castilla-La Mancha; Ex = Extremadura; Ga = Galicia; IB = Balearic Islands; Ma = Madrid; Na = Navarra. HEY = Herrold's Egg Yolk Medium with mycobactin J; LJ = Löwenstein-Jensen Medium with mycobactin J; 7H11 = OADC supplemented Middlebrook 7H11 Medium without mycobactin J; 7H11* = OADC supplemented Middlebrook 7H11 Medium with mycobactin J; 7H9+7H11 = OADC supplemented Middlebrook 7H9 Broth without mycobactin J, subcultured on 7H11; 7H9*+7H11 = OADC supplemented Middlebrook 7H9 Broth with mycobactin J, subcultured on 7H11. | ||||||||
Sevilla et al. BMC Microbiology 2007 7:18 doi:10.1186/1471-2180-7-18 |
||||||||