Table 1

SnaBI-SpeI PFGE classification of Map strains.

Country

PFGE profile

Locations

Host sp

No. of isolates (%)

No. of herds or flocks (%)

Other types in same herd/flock (No. of herds/flocks)

Primary Isolation

IS1311 type


Spain

1-1

BC, As, Ar, Na, Cat

Cattle

75 (32.33)

40 (30.53)

1–20 (1), 2-1 (6), 22-1 (1), 23-16 (1), 28–30 (1), 31-1 (1)

HEY, LJ, 7H11*

C type

*1–10

BC

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*1–20

Na

Cattle

1 (0.43)

1 (0.76)

1-1 (1), 31-1 (1)

HEY

C type

*1–27

BC

Cattle

3 (1.29)

1 (0.76)

HEY

C type

2-1

BC, As, Can, Ga, CL, Ar, Na, Cat, CM

Cattle

121 (52.16)

76 (58.02)

1-1 (6), 2–10 (2), 2–15 (1), 2–31 (1), 22-1 (1), 23-16 (2), 28–30 (1)

HEY, LJ, 7H11*

C type

2–5

As

Cattle

1 (0.43)

1 (0.76)

HEY

C type

2–10

Can, CL, Ex

Cattle

3 (1.29)

3 (2.29)

2-1 (2)

HEY

C type

*2–12

BC

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*2–14

Ga

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*2–15

BC, Ma

Cattle

3 (1.29)

3 (2.29)

2-1 (1), 23-16 (1)

HEY

C type

*2–25

CL

Cattle

2 (0.86)

1 (0.76)

HEY

C type

*2–31

CL

Cattle

1 (0.43)

1 (0.76)

2-1 (1)

HEY

C type

*20–27

BC

Cattle

2 (0.86)

2 (2.29)

HEY

C type

*21-1

Can

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*22-1

Ar

Cattle

1 (0.43)

1 (0.76)

1-1 (1), 2-1 (1)

HEY

C type

*23-16

BC

Cattle

7 (3.02)

7 (5.34)

1-1 (1), 2-1 (2), 2–15 (1)

HEY

C type

*24-19

Ma

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*27-26

BC

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*28–30

BC

Cattle

1 (0.43)

1 (0.76)

1-1 (1), 2-1 (1)

HEY

C type

*31-1

Na

Cattle

1 (0.43)

1 (0.76)

1-1 (1), 1–20 (1)

HEY

C type

*32-1

BC, Na

Cattle

2 (0.86)

2 (1.53)

HEY

C type

*33-1

BC

Cattle

1 (0.43)

1 (0.76)

HEY

C type

*40-1

CL

Cattle

1 (0.43)

1 (0.76)

HEY

C type

2-1

Ar

Sheep

1 (5.88)

1 (10.0)

LJ

C type

*25-23

Ar

Sheep

1 (5.88)

1 (10.0)

LJ

S type

*26-24

BC, Na

Sheep

3 (17.65)

2 (20.0)

LJ, 7H9*+7H11

S type

*30-11

BC

Sheep

2 (11.76)

2 (20.0)

39-17 (2), 39-21 (1), 41-32 (1)

7H11

S type

*35-29

BC

Sheep

1 (5.88)

1 (10.0)

7H11*, 7H9+7H11

S type

*36-18

BC

Sheep

1 (5.88)

1 (10.0)

38-22 (1), 39-17 (1)

7H11

S type

*38-22

BC

Sheep

1 (5.88)

1 (10.0)

36-18 (1), 39-17 (1)

7H11

S type

*39-17

BC, Na

Sheep

4 (23.53)

4 (40.0)

30-11 (2), 36-18 (1), 38-22 (1), 39-21 (1), 41-32 (1)

7H11

S type

*39-21

BC

Sheep

2 (11.76)

2 (20.0)

30-11 (1), 39-17 (1)

7H11

S type

*41-32

BC

Sheep

1 (5.88)

1 (10.0)

30-11 (1), 39-17 (1)

7H11

S type

2-1

BC

Goat

2 (28.57)

1 (20.0)

HEY, 7H11*

C type

15-1

An

Goat

1 (14.29)

1 (20.0)

34-28 (1)

7H11*, 7H9+7H11

C type

16-11

CL, An, IB

Goat

3 (42.86)

3 (60.0)

LJ, 7H9+7H11

S type

*34-28

An

Goat

1 (14.29)

1 (20.0)

15-1 (1)

7H9+7H11

S type

*37-1

CM

Deer

2 (100.0)

1 (100.0)

HEY

C type

2-1

CM

Wild Boar

1 (100.0)

1 (100.0)

HEY, 7H11*

C type


India

*29-1

Mathura

Sheep

2 (100.0)

1 (100.0)

HEY

B type

*29-1

Farah

Goat

5 (100.0)

1 (100.0)

HEY

B type


USA

2-1

Montana

Bison

3 (100.0)

1 (100.0)

LJ

B type


Details of SnaBI-SpeI multiplex profiles of the 269 Map isolates studied by PFGE: geographic origin, number of isolates, number of herds/flocks of the indicated host species, primary isolation media and IS1311 PCR-REA type. Profiles in boldface correspond to pigmented isolates. *novel profiles found in this study. Abbreviations: An = Andalucía; Ar = Aragón; As = Asturias; BC = Basque Country; Can = Cantabria; Cat = Cataluña; CL = Castilla y León; CM = Castilla-La Mancha; Ex = Extremadura; Ga = Galicia; IB = Balearic Islands; Ma = Madrid; Na = Navarra. HEY = Herrold's Egg Yolk Medium with mycobactin J; LJ = Löwenstein-Jensen Medium with mycobactin J; 7H11 = OADC supplemented Middlebrook 7H11 Medium without mycobactin J; 7H11* = OADC supplemented Middlebrook 7H11 Medium with mycobactin J; 7H9+7H11 = OADC supplemented Middlebrook 7H9 Broth without mycobactin J, subcultured on 7H11; 7H9*+7H11 = OADC supplemented Middlebrook 7H9 Broth with mycobactin J, subcultured on 7H11.

Sevilla et al. BMC Microbiology 2007 7:18   doi:10.1186/1471-2180-7-18

Open Data