Table 2

Sequences of primers used for the V-BiA-RE nanoplex PCR.

Gene

Primer Name

5'--------------------------------3'

GenBank Accession Number

Product Size


16S rRNA

16SrRNA-F

AGG GGA TAA CAC TTG GAA ACA

AB015233

1178 bp

16SrRNA-R

TTC GCG ACT CGT TG TAC TTC

ddl E. faecalis

Elis-F2

GGC CCT CTT TTA TCT GAA CGA

U00457

734 bp

Elis-R3

GCG ACT TAA GCC ACT TCC AT

ddl E. faecium

Ecium-F

CGC AGA GCA TGA AGT GTC CA

AF550665

557 bp

Ecium-R2

CTT CTC GGT TTT CTG CTT TTG TA

VanA

vanA-F

TTG GGG GTT GCT CAG AGG AG

X56895

931 bp

vanA-R

CTT CGT TCA GTA CAA TGC GG

VanB

vanB-F

AAT GCG GGG AGG ATG GTG CG

AF550667

446 bp

vanB-R

GAT GCG GAA GAT ACC GTG GC

VanC

vanC-F2

GCA GGT TCT GCC TTA TGT ATG AA

AF162694, AY033764, L29638

339 bp

vanC-R

ATG AAA TGG CGT CAC AAG CA

VanD

vanD-F

CGT ATG TGG GAT GCG ATA TTC AA

AF130997

230 bp

vanD-R2

CTT CGA TTG CTG CCT GCA GTT

aacA-aphD

acph-F1L

GAT TTG CCA GAA CAT GAA TTA CAC GA

AY602207

156 bp

acph-R1L

CAT AAC CAC TAC CGA TTA TTT CAA T

Internal Control (ctxA)

IC-F

AAC TCA GAC GGG ATT TGT TAG GC

AF510996

300 bp

IC-R

TCT CTG TAG CCC CTA TTA CGA TGT


Yean et al. BMC Microbiology 2007 7:112   doi:10.1186/1471-2180-7-112

Open Data