Table 1

Bacterial species and strains used in this study and results of V-BiA-RE nanoplex PCR.

No.

Reference strains

16S rRNAa

ddl-E. faecalis

ddl-E. faecium

vanA

vanB

vanC

vanD

aacA-aphDb

Internal control


1.

E. faecium LMG 16004c

+

-

+

+

-

-

-

-

+

2.

E. durans LMG 16172c

+

-

-

+

-

-

-

-

+

3.

E. faecium LMG 16192c

+

-

+

+

-

-

-

-

+

4.

E. faecalis LMG 8222c

+

+

-

-

-

-

-

-

+

5.

E. faecalis LMG 17122c

+

+

-

-

-

-

-

-

+

6.

E. faecalis LMG 16216c

+

+

-

-

+

-

-

+

+

7.

E. faecium LMG 16200c

+

-

+

-

-

-

-

-

+

8.

E. raffinosus LMG 12172c

+

-

-

-

-

-

-

-

+

9.

E. mundti LMG 12308c

+

-

-

-

-

-

-

-

+

10.

E. hirae LMG 6399c

+

-

-

-

-

-

-

-

+

11.

E. avium, LMG 10744c

+

-

-

-

-

-

-

-

+

12.

E. faecium BM 4339 d

+

-

+

-

-

-

+

+

+

13.

E. casseliflavuse

+

-

-

-

-

+

-

-

+

14.

S. aureusf

-

-

-

-

-

-

-

-

+

15.

Streptococcus spp. Group Af

-

-

-

-

-

-

-

-

+

16.

Streptococcus spp. Group Bf

-

-

-

-

-

-

-

-

+

17.

Streptococcus spp. Group Gf

-

-

-

-

-

-

-

-

+

18.

Streptococcus spp. Group Ff

-

-

-

-

-

-

-

-

+

19.

Bacillus spp.f

-

-

-

-

-

-

-

-

+

20.

Listeria spp.f

-

-

-

-

-

-

-

-

+

21.

Corynebacterium spp.f

-

-

-

-

-

-

-

-

+

22.

Gardnerella spp.f

-

-

-

-

-

-

-

-

+

23.

Lactobacillus spp.f

-

-

-

-

-

-

-

-

+

24.

E. coli (EHEC)f

-

-

-

-

+

-

-

-

+

25.

E. coli (EPEC)f

-

-

-

-

-

-

-

-

+

26.

E. coli (ETEC)f

-

-

-

-

+

-

-

-

+

27.

V. cholerae (O1 classical)f

-

-

-

-

-

-

-

-

+

28.

V. cholerae O139 f

-

-

-

-

-

-

-

-

+

29.

V. mimicusf

-

-

-

-

-

-

-

-

+

30.

V. cincinnatiensisf

-

-

-

-

-

-

-

-

+

31.

V. furnissiif

-

-

-

-

-

-

-

-

+

32.

V. parahaemolyticusf

-

-

-

-

-

-

-

-

+

33.

S. typhif

-

-

-

-

-

-

-

-

+

34.

S. sonneif

-

-

-

-

-

-

-

-

+

35.

S. entericaf

-

-

-

-

-

-

-

-

+

36.

S. dysenteriaef

-

-

-

-

-

-

-

-

+

37.

S. boydiif

-

-

-

-

-

-

-

-

+

38.

C. freundiif

-

-

-

-

-

-

-

-

+

39.

Y. enterocoliticaf

-

-

-

-

-

-

-

-

+

40.

P. mirabilisf

-

-

-

-

-

-

-

-

+

41.

P. aeruginosaf

-

-

-

-

-

-

-

-

+

42.

K. pneumoniaef

-

-

-

-

-

-

-

-

+

43.

P. Shigelloidesf

-

-

-

-

-

-

-

-

+


aEnterococcus genus

b Bifunctional aminoglycoside resistant genotype

c Reference strains from Belgian Co-ordinated Collections of Micro-organisms (BCCM), Ghent, Belgium

d Kindly provided by Professor. Patrice Courvalin and Dr. Bruno Perichon, Institut Pasteur, Paris, France

e Obtained from Institute for Medical Research, Malaysia

f Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia. '+' is positive; '-' is negative by V-BiA-RE PCR

Yean et al. BMC Microbiology 2007 7:112   doi:10.1186/1471-2180-7-112

Open Data