|
ALO-mediated lysis of human monocytes by B. anthracis is inhibited by 10% FBS. Percent viable THP-1s in the presence of B. anthracis Sterne strain 7702 (ALO+), UT231 (ALO-) and the complemented over-producer, UT231 (pUTE544) (ALO++) at an MOI of 1000 in the presence of 10 % FBS. At various time points, aliquots were mixed with 0.25 % trypan blue to measure viability. Native ALO expressed by 7702, at all time points, and UT231 (pUTE544), at 5 min, is inhibited by cholesterol present in heat-inactivated 10% FBS. Results are average ± standard deviations of two experiments. |
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| % Viable THP-1 monocytes in presence of 10% FBS |
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| Sterne 7702 |
UT231 |
UT231(pUTE544) |
Control (no bacteria) |
|
|
|
||||
| 5 min |
100 |
100 |
100 |
100 |
| 30 min |
100 |
100 |
0 |
99 ± 0 |
| 60 min |
95 ± 1 |
98 ± 2 |
0 |
99 ± 0 |
| 120 min |
92 ± 3.2 |
96 ± 1 |
0 |
97 ± 1 |
Mosser and Rest BMC Microbiology 2006 6:56 doi:10.1186/1471-2180-6-56 |
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