Table 1 |
|||||||
|
Purification of mutacin H-29B by hydrophobic chromatography. |
|||||||
|
Step |
Volume (mL) |
Activity (AU/mL) |
Total Protein (mg) |
Total activity (AU. 103) |
Specific activity (AU/mg) |
Yield (%) |
Purification (fold) |
|
|
|||||||
|
Culture supernatant |
1250 |
6400 |
10000 |
8000 |
800 |
100 |
1 |
|
XAD-7 Amberlite |
300 |
6400 |
97.5 |
1920 |
20000 |
24 |
25 |
|
Sep-Pak C18 |
95 |
12800 |
1.9 |
1216 |
6.4 × 105 |
15.2 |
800 |
|
C18 RP-HPLC |
1 |
6.4 × 105 |
0.1 |
640 |
6.4 × 106 |
8 |
8000 |
|
|
|||||||
|
Nicolas et al. BMC Microbiology 2006 6:36 doi:10.1186/1471-2180-6-36 |
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