Table 2

Sensitivity of real-time PCR for detection of a serial 10-fold diluted E. sakazakii DNA in presence of IAC

Strain 29544
 Strain ATCC 51329

Nb. of genome copies/PCR
 CT value (target)
 CT value (IAC)
 CT value (target)
 CT value (IAC)
106
 19.60 ± 0.15
 -
 23.79 ± 0.23
 -
105
 23.75 ± 0.16
 -
 28.24 ± 0.50
 -
104
 27.99 ± 0.98
 -
 32.11 ± 0.49
 -
103
 32.86 ± 0.03
 > 38
 37.04 ± 0.71
 39.44 ± 1.20
102
 36.27 ± 0.68
 40.85 ± 1.11
 40.56 ± 0.86
 41.10 ± 0.99
50
 37.67 ± 0.21
 40.53 ± 0.85
 41.49 ± 0.75
 40.32 ± 0.22
25
 38.42 ± 0.46
 40.24 ± 1.53
 42.09 ± 0.97
 41.20 ± 0.62
10
 38.99 ± 1.82
 37.14 ± 1.66
 43.73 ± 1.41
 41.29 ± 1.84
5
 40.79 ± 1.41
 38.77 ± 2.95
 > 43.7
 40.22 ± 1.77
1
 > 44
 40.46 ± 0.87
 > 45
 39.86 ± 0.27

CT values are means ± standard deviation obtained for three replicates

Derzelle and Dilasser BMC Microbiology 2006 6:100   doi:10.1186/1471-2180-6-100