Table 1

Proteins showing differential expression on 2-D gels.


Differential expression1



Spot no.
 Protein
 Spermine (1 mM)
 Spermidine (10 mM) pH 8.5
 Known or predicted function


pH 7.0
 pH 8.5

1
 PykF
 0.34 ± 0.09
 (+)
 (+)
 Pyruvate kinase
2
 PykF
 0.39 ± 0.06
 (+)
 (+)
 Pyruvate kinase
3
 GlpK
 0.68 ± 0.09
 0.63 ± 0.12
 0.50 ± 0.09
 Glycerol kinase
4
 SerS
 0.83± 0.08
 0.60 ± 0.1
 0.60 ± 0.1
 Serine-tRNA ligase
5
 ThrC
 -0.34 ± 0.06

Threonine synthase
6
0.51 ± 0.03
 0.81 ± 0.07

7
 TnaA
0.92 ± 0.06
 0.87 ± 0.09
 Tryptophanase
8
 TnaA
0.53 ± 0.07
 0.77 ± 0.07
 Tryptophanase
9
 MalE
 -1.04 ± 0.05

Maltose-binding, periplasmic
10
 MalE
 -1.0 ± 0.00

Maltose-binding, periplasmic
11
 MalE


Maltose-binding, periplasmic
12
 MalE
 -0.74 ± 0.08

Maltose-binding, periplasmic
13
 Asd
 -0.54 ± 0.10
-0.42 ± 0.07
 Aspartate semialdehyde dehydrogenase
14
 FabB
 (-)
 (-)
 -0.64 ± 0.11
 Beta-Ketoacyl-ACP synthase I
15
 DeaD
 0.27 ± 0.03
 0.41 ± 0.08
ATP-dependent RNA helicase
16
 TktA
 -0.46 ± 0.11
 (-)
 -0.48 ± 0.07
 Transketolase
17
 OmpC
 (+)
 0.56 ± 0.05
Outer membrane protein C
18
 OmpF
 0.96 ± 0.03
 0.74 ± 0.15
Outer membrane porin
19
 OmpF
 0.56 ± 0.05
 0.90 ± 0.08
Outer membrane porin
20
 Pta
 (-)
 -0.50 ± 0.15
 (-)
 Phosphate aceyltransferase
21
-0.47 ± 0.06
 (-)
 (-)
22
 MglB

0.23 ± 0.02
 Galactose-binding protein
23
0.69 ± 0.11
 (+)

24
 MalM
 -59 ± 0.10

Maltose periplasmic protein
25
 SucB
 -0.25 ± 0.02
 -0.89 ± 0.08
Dihydrolipoamide Succinyltransferase
26
 GlnS
 (-)
 -0.59 ± 0.11
 -0.60 ± 0.1
 Glutaminyl-tRNA synthetase
27
 NanA
0.49 ± 0.12
Putative N-acetylmanosamine-6-phosphate 2-epimerase
28
 Lpd
 (-)
 -0.79 ± 0.1
 -0.69 ± 0.1
 Dihydrolipoamide dehydrogenase
29
 Lpd
 (-)
 -0.55 ± 0.1
 -0.72 ± 0.17
 Dihydrolipoamide dehydrogenase
30
 Lpd
 (-)
 -0.58 ± 0.1
 -0.27 ± 0.05
 Dihydrolipoamide dehydrogenase
31
 FabE
-0.24 ± 0.04
 -0.82 ± 0.07
 Acetyl-CoA carboxylase
32
 RpsB
 (-)
 -0.26 ± 0.05
30S ribosomal subunit protein S2
33
 YrdA
0.26 ± 0.04
 0.44 ± 0.12
34
 OmpA
0.27 ± 0.04
 0.43 ± 0.09
 Outer membrane protein A
35
 RpsF
 -0.22 ± 0.01
 -0.85 ± 0.09
 -0.50 ± 0.12
 30S ribosomal subunit protein S6
36
 RpsF
 -0.22 ± 0.01

30S ribosomal subunit protein S6
37
 Gpt
 0.18 ± 0.03
 0.40 ± 0.08
 0.58 ± 0.1
38
 TpiA
 -0.28 ± 0.03
 -0.57 ± 0.06
 (-)
 Triosephosphate isomerase
39
 RapA (HepA)
 -0.61 ± 0.02
 -0.76 ± 0.09
 -0.63 ± 0.11
 RNA polymerase binding protein
40
 YfiD
 -0.27 ± 0.02
 -0.21± 0.02
 -0.37 ± 0.03
 Pyruvate formate-lyase homolog
41
 GapA
 -0.51 ± 0.1
 -0.87 ± 0.06
 -0.50 ± 0.13
 Glyceraldehyde 3-phosphate dehydrogenase A
42
 AtpA
 -0.27 ± 0.07

ATP synthase subunit alpha
43
-0.74 ± 0.02
 (-)
 (-)
44
 GuaB

-0.75 ± 0.1
 Inosine-5'-monophosphate dehydrogenase

1Relative differential expression of protein, shown as LDE ± standard error (n = 9), was determined as described under Materials and Methods. Symbols: (+), induced; (-) repressed, representing spots that showed differential expression based on visual inspection, although not quantifiable by the Z3 software.

Yohannes et al. BMC Microbiology 2005 5:59   doi:10.1186/1471-2180-5-59