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Table 4
Bacterial strains and plasmids used or created in this study
Strains	Genotype	Source or Reference

Escherichia coli

XL1-Blue	K12 supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac^-F'[proAB⁺lacI^qlacZΔM15 Tn10(tet^R)]	Laboratory collection
TG1	K12 supE hsdΔ5 thi Δ (lac-proAB) F'[traD36 proA⁺proB⁺lacI^qlacZΔM15]	Laboratory collection
BL5(DE3)	F^-	Invitrogen
EM9	P_T7-map (on chromosome)	Chiron Culture Collection Centre
ECIP8003	F^-pIPP8003	This work
ECIP8004	F^-pIPP8004	This work
ECHP1005	EM9 pHPP1017	This work
ECHP1006	EM9 pHPP1018	This work
ECHP1007	EM9 pBAD	This work

Bacillus subtilis

168	trpC2	Laboratory collection
BFS4611	trpC2 yflG::lacZ	Functional analysis project^a
BFS817	trpC2 yvoA::lacZ	Functional analysis project^a
BSHP7042	trpC2 map-lacZ	This work
BSHP7045	trpC2 pDG148-map	This work
BSHP7046	trpC2 map::lacZ pDG148-map	This work
BSHP7037	trpC2 map::lacZ pDG148-yflG	This work
BSHP7038	trpC2 pDG148-yflG	This work
BSHP7043	trpC2 yflH::lacZ	This work
BSIP8001	trpC2 amyE::gfp-map	This work
BSIP8002	trpC2 amyE::gfp-yflG	This work
BSIP8003	trpC2 yvoA::[(lacZ-ery)::pEC23(Kan)]	This work
BSIP8004	trpC2 yflG::lacZ yvoA::[(lacZ-ery)::pEC23(Kan)]	This work

Plasmids	Description	Source or Reference

pET24b(+)	expression vector, Kan^R	Novagen
pBAD-abg	genetic rescue vector, Amp^R	[43]
pDG148	expression vector, Amp^RKan^R	[44]
pJM783	cloning vector, Cm^R	[45]
pSG1729	N-terminal GFP fusion vector, Amp^RSpec^R	[42]
pEC23	integrative plasmid, Kan^R	M. Simon and P. Stragier, unpublished
pIPP8003	pET24b map	This work
pIPP8004	pET24b yflG	This work
pHPP1017	pBAD map	This work
pHPP1018	pBAD yflG	This work
pHPP7017	pJM783 map-lacZ	This work
pHPP7018	pJM783 yflH::lacZ	This work
pHPP7021	pDG148 map	This work
pHPP7022	pDG148 yflG	This work
pHPP7025	pJM783 map::lacZ	This work
pIPP8001	pSG1729 map	This work
pIPP8002	pSG1729 yflG	This work

^aThis strain has been constructed during the frame of the project for the functional characterization of the genome of B. subtilis in Japan [46].
You et al. BMC Microbiology 2005 5:57 doi:10.1186/1471-2180-5-57

Table 4