Table 1

Detailed analysis of the effects of preincubating cultures of A. awamori with 3,5-DCP, Cr6+ and Zn2+ on [Ca2+]c response to 5 mM external CaCl2

Toxicant

Features of [Ca2+]C Signatures


A

LT50

FRL (%) 5 min pre-incubation

RT (%) 5 min pre-incubation

FRL (%) 30 min pre-incubation

RT (%) 30 min pre-incubation


3,5-DCP

0.112

-

111 ± 8.4 (↑)

111 ± 8.4 (↑)

106 ± 7.6 (-)

98 ± 1.3 (-)

(mgl-1)

11.2

-

120 ± 4.8 (↑)

125 ± 4.6 (↑)

127 ± 29.7 (-)

134 ± 31.9 (↑)

112

289 ± 17.6 (↑)

292 ± 6.6 (↑)

416 ± 23.2 (↑)

416 ± 16.6 (↑)

Cr6+

15

-

-

-

-

-

(mgl-1)

120

-

-

-

-

-

260

-

-

-

-

-

Zn2+

180

-

106 ± 9.2(-)

127 ± 4.6 (↑)

97 ± 6.9(-)

92 ± 1.9(↓)

(mgl-1)

350

-

153 ± 13.6(↑)

167 ± 14.4(↑)

149 ± 11.4(↑)

143 ± 10.2(↑)

700

164 ± 30.7(↑)

179 ± 26.9(↑)

245 ± 16.5(↑)

261 ± 9.6(↑)

1300

169 ± 14.8(↑)

182 ± 10.4(↑)

169± 12.6(↑)

176 ± 16.5(↑)


Note: – no effect compared with untreated control), ↑ increase in parameter, ↓ decrease in parameter, A – amplitude, LT50 – Length of transient, FRL – Final Ca2+ resting level, RT – recovery time. A and LT50 are shown only for 5 min incubation. Results represent mean ± SD. The % values represent the absolute value of the parameter not the relative increase compared with control. N = 6

Kozlova et al. BMC Microbiology 2005 5:40   doi:10.1186/1471-2180-5-40

Open Data