Table 4 |
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|
Primers for PCR |
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|
Gene assignment in strain NEM316 |
Gene assignment in strain 2603VR |
Putative island |
Primer Pairs |
|
|
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|
gbs0001 |
sag0001 |
- |
5'-gtagctgatagtcctggc-3' and 5'-agtccccaactaaagcgc-3' |
|
gbs0045-46 |
sag0046 |
MME |
5'-aaatgggacacgtacgg-3' and 5'-attgccgccatctcaggg-3' |
|
gbs0217 gbs0227 |
sag0224 sag0234 |
I |
5'-caagcctttaatgctcgc-3' and 5'-aactgaaattccaatcgcc-3' 5'-tcatcgcgaaaatatggag-3' and 5'-cggtcttttagaaactgtgtcc-3' |
|
gbs0247 |
sag0254 |
II |
5'-gacttatttcaagtttatgg-3' and 5'-acccttatatacgacagc-3' |
|
gbs0367 gbs0388 gbs0393 |
- |
pNEM316-1 (islands III, VII and VIII) |
5'-atcgatttaggattcatgcc-3' and 5'-caacattcgcaaaataagcc-3' 5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagcg-3' 5'-tcacccctgagacgtttacc-3' and 5'-gatcgtaaccacggtttgct-3' |
|
gbs0467 |
sag0430 |
IV |
5'-attgatagatcttacttgcg-3' and 5'-tgatgcaatagctattggc-3' |
|
gbs0589 gbs0598 |
sag0610 sag0617 |
V |
5'-cagggtgttcaaggctacc-3' and 5'-caagcttacgcacccaag-3' 5'-ctttcctaaaacatatttgg-3' and 5'-atatggtaaaaacttaaggc-3' |
|
gbs0628 gbs0660 |
sag0645 sag0685 |
VI |
5'-tagctcagtttgcgactgg-3' and 5'-ccaacttttgcatctgctg-3' 5'-aattcttgattgatgagcg-3' and 5'-tcagctttaatcaattccc-3' |
|
- |
sag0915 |
Tn916 |
5'-aagaccaaaagtggcgaac-3' and 5'-gcctttggattcattcctg-3' |
|
gbs1050 gbs1073 |
sag1015 sag1038 |
IX |
5'-agcagttacttgatttgcc-3' and 5'-tcctgaattagctagtcgc-3' 5'-tctgcttgagataactccc-3' and 5'-caatagcagttatcaaaggg-3' |
|
gbs1120 gbs1125 gbs1135 gbs1143 gbs1145 |
- |
X |
5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagc-3' 5'-tcgacgtgttttacggttg-3' and 5'-accgaagagatgatgacgac-3' 5'-gggccacactagaaactgc-3' and 5'-aaatccttcatcgctcctg-3' 5'-tcacccctgagacgttacc-3' and 5'-gatcgtaaccacggtttgct-3' 5'-tctctcggcgttattgtcc-3' and 5'-acaaaagcacaagcgactg-3' |
|
gbs1306 gbs1313 |
sag1233 sag1246 |
XII |
5'-ctttactggcttcacttgg-3' and 5'-gttgatacaggcattgagc-3' 5'-gattactctaccagtgagg-3' and 5'-agaatagtctgcttcaccc-3' |
|
gbs1987 gbs2008 |
sag2029 sag2053 |
XIII |
5'-ctgacaattgctttgtttcg-3' and 5'-ggctaacccaaatgtaccg-3' 5'-gctcctctgattaatgccc-3' and 5'-caagctcttgttcggttgc-3' |
|
gbs2082 |
sag2123 |
XIV |
5'-tttctgggaaaaatcagtgg-3' and 5'-ttttcccgaacaaatgatg-3' |
|
|
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Herbert et al. BMC Microbiology 2005 5:31 doi:10.1186/1471-2180-5-31 |
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