Table 2

Comparison of three PCR assays and virus isolation from koi carp exposed to KHV
Sample
 TK PCR
 Gilad PCR
 Gray PCR
 Virus Isolation
 History of the koi carp
1
 +
 -
 -
 +
 sick fish with clinical symptoms
2
 -
 -
 -
 -
 survivor (unknown time lap after exposure).
3
 -
 -
 -
 -
 survivor (unknown time lap after exposure) from a tank with mortality.
4
 +
 -
 -
 +
 survivor (unknown time lap after exposure) from a tank with mortality.
5
 -
 -
 -
 -
 survivor moved to 30°C, then to 24°C. no mortality in the tank.
6
 +
 -
 -
 +
 survivor moved to 30°C, then moved to 24°C. 25% mortality in the tank.
7
 -
 -
 -
 -
 naïve fish that lived at 16°C. No mortality in the tank.
8
 +
 +
 +
 +
 fish from tank with mortality.
9
 +
 -
 -
 +
 fish from tank with mortality.
10
 -
 -
 -
 Pooled
 survivor, 2 years after experimental infection
11
 -
 -
 -
 -
 survivor, 2 years after experimental infection
12
 -
 -
 -
 Pooled
 survivor, 1 year after experimental infection
13
 -
 -
 -
 -
 survivor, 1 year after experimental infection
14
 -
 -
 -
 Pooled
 survivor, 2 months after experimental infection
15
 -
 -
 -
survivor, 2 months after experimental infection
16
 +
 -
 -
 +
 survivor, 2 months after experimental infection
17
 +
 -
 -
survivor, 2 months after experimental infection
18
 +
 -
 -
 +
 survivor from tank with disease- two weeks post infection.
KF+
 +
 +
 +
 +
 KF-1 cell line infected with KHV- Positive control.
KF-
 -
 -
 -
 -
 KF-1 cell line- Negative control.

Bercovier et al. BMC Microbiology 2005 5:13   doi:10.1186/1471-2180-5-13