Table 1

Clinical data and phenotypic and genotypic identification results of the Klebsiella pneumoniae and Enterobacter aerogenes isolates used in this study

Strain

Sample

Motilityb

Ornithine decarboxylaseb

API Code

API Identificationc

Vitek2d Phoenixd

tDNA-PCRe

Clonef


Group 1

LBV268

+

+

5305773

E. aerogenes

E. aerogenes

E. aerogenes

BEI

MN1

Aspirate

+

+

5305773

E. aerogenes

NT

E. aerogenes

BEII

BEI 166

+

+

5305773

E. aerogenes

NT

E. aerogenes

BEI

BEII 169

+

+

5305773

E. aerogenes

NT

E. aerogenes

BEII

Group 2

BG

Blood culture

-

-

5005763

K. pneumoniae/K. terrigena

K. pneumoniae

K. pneumoniae

K1

DHJ2

Aspirate

-

-

5215773

K. pneumoniae

K. pneumoniae

K. pneumoniae

K2

Group 3

GA1

Wound

(-)

(-)

5205773

Weak

NT

E. aerogenes

BEI

GA2

Urine

(-)

(-)

5205773

Weak

NT

E. aerogenes

BEI

GA3

Urine

(-)

(-)

5205773

Weak

K. pneumoniae

E. aerogenes*

BEI

MN2

Ascites

(-)

(-)

5205773

Weak

K. pneumoniae

E. aerogenes

BEI

MN3

Urine

(-)

(-)

5205773

Weak

NT

E. aerogenes*

BEI

VGM

Sputum

(-)

(-)

5205773

Weak

K. pneumoniae

E. aerogenes

BEII-related

DHJ1

Urine

(-)

(-)

5205753

No identification

NT

E. aerogenes*

BEII-related

DHJ3

Blood culture

(-)

(-)

5205753

No identification

NT

E. aerogenes

BEII-related

RA

Throat isolate

(-)

(-)

5204673

K. terrigena

NT

E. aerogenes*

Non-related

DBH

Urine

(-)

(-)

5204673

K. terrigena

NT

E. aerogenes*

BEI


a: Data for genuine E. aerogenes isolates are presented first (group 1). Clinical isolate LBV268 was used as control for analysis on automated phenotypical identification systems. The isolates BEI 166 and BEII 169 were shown previously [4] to belong to the two major E. aerogenes clones (BEI and BEII) in Belgium. Group 2 presents data for genuine K. pneumoniae. Clinical isolate BG was used as control for analysis on automated phenotypical identification systems. The third group presents the data for the phenotypically aberrant E. aerogenes isolates.

b: +, positive; -, negative; (-), negative after standard incubation time, only positive after subculturing and/or retesting with prolonged incubation periods.

c: Weak: Weak identification with possibilities: E. aerogenes, K. pneumoniae or R. planticola.

d: NT: not tested.

e: * indicates that identification was confirmed by 16S rDNA sequence analysis.

f: Clonal relationships were determined using RAPD-analysis.

Claeys et al. BMC Microbiology 2004 4:49   doi:10.1186/1471-2180-4-49

Open Data