Figure 1.

Overview of the e14 genome. The genetic functions of a generic lambdoid bacteriophage genome (brown rectangle) are shown above displayed with a transcriptional map (black arrows). In the middle, the section of the E. coli K-12 genome that contains e14 (gray rectangle) is shown with ORFs denoted by rectangular arrows oriented in the direction of transcription (green – host genes; red – e14 genes that are likely nonfunctional; black – e14 genes that are known to be functional; blue – e14 genes whose functionality cannot be assessed at present; parentheses indicate the boundaries of the P-invertable element). Small black arrows above the e14 map denote putative promoters, vertical lines denote putative terminators and small black squares putative operators. The yellow regions between the lambdoid and e14 maps indicate regions where e14 has homology to at least one known member of the lambdoid phage family (see text for details). Below, colored rectangles mark regions of highest homology between e14 and various known phages and prophages with regions of greater similarity closer to the e14 map (these are not meant to show all known homologies, only the closest ones); CPS-53 is a defective prophage in E. coli K-12, CP-933H is prophage in E. coli EDL933 and CP073-5, Sti4b, and Sti8 are provisional names for prophages in E. coli CFT073 and S. typhi CT18 (Supplementary Material of Ref. [39]).