Table 1

Plasmids and E. coli strains used in this study.

Plasmid

Description

Reference


pBluescript

Cloning vector, ApR

Stratagene

pDD515

RSF1010 ori; a mobilizable broad host range vector, CmR

12

pDD559

pSR658 with vapDHi in frame with wild-type LexA DNA-binding domain (DBD)

This work

pDD560

pTrcHisA with vapDHi

This work

pDD561

pSR659 with vapDHi in frame with mutant LexA DBD

This work

pDD562

pBluescript with vapDHi region

This work

pDD563

pDD652 with an aph(3')II gene interrupting vapDHi

This work

pDD564

pDD515 with a full-length vapDHi region for transcomplementation

This work

pDD577

pSR658 with a truncated vapDHi in frame with the wild-type LexA DBD

This work

pDD594

pDD515 with a truncated vapDHi region for transcomplementation

This work

pDD672

pDD515 with the vapXHi antitoxin for rescue

This work

pSR658

Wild-type LexA DBD fusion vector, TcR

15

pSR659

Mutant LexA DBD fusion vector, ApR

15

pTrcHisA

Cloning vector, ApR

Invitrogen


Strain

Description

Source


DD12

Conjugal host strain

12

DH5α

Cloning strain

Promega

SU101

Homodimerization reporter strain

14

SU202

Heterodimerization reporter strain

14


Daines et al. BMC Microbiology 2004 4:30   doi:10.1186/1471-2180-4-30

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