Table 1 |
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|
Primer sequences for sequencing of merA genes and for merA-TGGE. |
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|
mer primers |
Direction |
Nucleotide sequence (5' to 3') |
|
|
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|
primers used for sequencing |
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|
T1 |
Forward |
GTCTGGATCGGCAACTTGA |
|
P1 |
Forward |
GGCTATCCGTCCAGCGTCAA |
|
A1 |
Forward |
ACCATCGGCGGCACCTGCGT |
|
A5 |
Reverse |
ACCATCGTCAGGTAGGGGACCAA |
|
A7 |
Reverse |
TGGCGCATTGACAGTBGACCC |
|
A9 |
Forward |
GAAYTGGATCCAGACGGCKG |
|
A10 |
Reverse |
GATCATGATCTTGGACGGCACACA |
|
D1 |
Reverse |
TCATGGCAAACTCTCCGC |
|
TGGE primers (GC-clamp in italics) |
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|
GC-merAf |
Forward |
CCCGCCGCCCCGCCCGCCGCCCCGCCCCGCCGCCCGCCTTGGAGAACGTGC |
|
merAr |
Reverse |
ACGTCCTTGGTGAAGGTCTG |
|
|
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|
Felske et al. BMC Microbiology 2003 3:22 doi:10.1186/1471-2180-3-22 |
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