Figure 1.

Effect of PCR annealing temperature (between 46°C and 64°C) on merA amplification efficiency with primers GC-merAf and merAr. The ethidium bromide-stained agarose gels show different annealing temperature dependencies for merA PCR products of representative isolates. (1) Ps. putida KT2442::mer73 [29] had its optimum al low annealing temperature; (2) P. putida Spi4 and most other strains tested worked best at medium annealing temperature; (3) P. stutzeri Ibu8 failed at low annealing temperatures but worked well at high temperatures.