Figure 5.

Growth of mutants from the mtn region with MTR as sole sulfur source. Panel A: ED1 minimal medium plate with 1 mM IPTG containing 0.2 mM MTR as sole sulfur source WT, metI (BSIP1143), mtnS (BSHP7010), mtnK (BFS1850), mtnU (BFS1851), mtnV (BSHP7020), mtnW (BSHP7014), mtnX (BFS1852), mtnY (BSHP7016) and mtnZ (BFS1853) were inoculated for over-night growth at 37°C. No growth of mtnS, mtnK, mtnW and mtnY is represented by an example of absence of growth around a disc with MTR of mtnY mutant in panelB. Normal growth of mtnV and mtnX is represented by an example of normal growth around a disc with MTR of mtnV mutant in panel C. The partial growth of the mtnZ mutant is illustrated by its growth around a disc with MTR in panel C. Panel B: The mtnY strain (BSHP7016) was inoculated on ED1 minimal medium plate with no added sulfur source. 10 μl of methionine (met) or MTR was put on paper discs and the plate was incubated over-night at 37°C. Panel C: The mtnV strain (BSHP7020) was inoculated on ED1 minimal medium plate with no added sulfur source. 10 μl of methionine (met) or MTR was put on paper discs and the plate was incubated over-night at 37°C. Panel D: The mtnZ strain (BFS1853) was inoculated on ED1 minimal medium plate with no added sulfur source. 10 μl of methionine (met) or MTR was adsorbed on paper discs and the plate was incubated over-night at 37°C. Methionine was used as a control.

Sekowska and Danchin BMC Microbiology 2002 2:8   doi:10.1186/1471-2180-2-8
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