Research article

First isolation of the enterohaemorrhagic Escherichia coli O145:H- from cattle in feedlot in Argentina

Nora L Padola¹ , Marcelo E Sanz¹ , Paula MA Lucchesi¹ , Jesús E Blanco² , Jorge Blanco² , Miguel Blanco² , Analía I Etcheverría¹ , Guillermo H Arroyo¹ and Alberto E Parma¹

¹  Laboratorio de Inmunoquímica y Biotecnología, Animal Health Department, Universidad Nacional del Centro-CIC, 7000 Tandil, Argentina

²  Laboratorio de Referencia E. coli Universidad Stgo. de Compostela, Lugo, España

author email corresponding author email

BMC Microbiology 2002, 2:6doi:10.1186/1471-2180-2-6

Published:	8 April 2002

Abstract

Background

Enterohaemorrhagic Escherichia coli (EHEC) is considered to be common cause of haemorrhagic colitis (HC), thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome (HUS) in humans. In a previous paper, we have demonstrated that EHEC are commonly found in the intestines of livestock. Infections in humans are, in part, a consequence of consumption of undercooked meat or raw milk. Argentina has one of the highest records of HUS (300–400 cases/year; 22/100,000 children under 4 years of age). The aim of this work is to communicate the first isolation of O145:H-from cattle in this country and characterize the virulence cassette, providing useful information to evaluate the risk of foodborne transmission of this emergent non-O157:H7 serotype.

Results

EHEC O145:H- was isolated from cattle in an Argentinian feedlot. Pheno- and genotype of nine strains were characterized, corresponding to several virulence cassettes: VT2⁺eaeA⁺ Mp⁺ (n = 5), VT2⁺eaeA⁺ (n = 1), VT1⁺eaeA⁺ Mp⁺ (n = 2), and VT1⁺eaeA⁺ (n = 1). Strains isolated from the same animal were considered only when they showed a different virulence pattern. The clonal relationship was studied by RAPD. Strains were distributed in two RAPD profiles, which corresponded to the presence of either, VT1⁺ or VT2⁺ genotype. No difference was detected by RAPD analysis between Mp⁺ or Mp^- strains.

Conclusions

This was the first isolation of EHEC O145:H- serotype in Argentina enlarging the list of non-O157:H7 serotypes isolated from cattle in this country by us. All O145:H-strains carried several virulence factors which allow us to predict their potential ability to develop haemolytic uraemic syndrome in humans.