Table 1 |
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Strains, plasmids, and phages. |
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Strains, plasmids and phages |
Parent |
Genotype or Phenotype |
Source |
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|
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Strains |
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|
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|
MC4100 |
F-araD139 (argF-lac) U169 rpsL150 relA1 flb5301 deoC1 ptsF25 rbsR |
[38] |
|
|
PC2 |
MC4100 |
fnr |
[4] |
|
|
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Plasmids |
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|
|
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|
pPC25 |
pDMS |
[4] |
|
|
pJA250 |
dmsA-lacZ TTGATaccgAACAA |
this study |
|
|
pJA303 |
dmsA-lacZ TTGATaccgATCAA |
this study |
|
|
pJA450 |
dmsA-lacZ TTGTTaccgATCAA |
this study |
|
|
pJA257 |
dmsA-lacZ TTAGTaccgAACAA |
this study |
|
|
pJA304 |
dmsA-lacZ ----Δ----accgATCAA |
this study |
|
|
pJA448 |
dmsA-lacZ TTGATaccgAACAAA |
this study |
|
|
pJA449 |
dmsA-lacZ TTGATaccgAACAAC |
this study |
|
|
pRS415 |
lacZ lacY+lacA+ |
[35] |
|
|
pRS1274 |
lacZ lacY+lacA+ |
[35] |
|
|
pBluescript |
Stratagene |
||
|
pBSDMS4 |
pBluescript |
dmsA' |
this study |
|
pGEM-11Zf |
Promega |
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|
pSB10 |
pGEM-11Zf |
dmsA -127 to -13 |
this study |
|
|
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Phage |
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|
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M13mp19-100 |
M13mp19 |
dmsA' |
laboratory stock |
|
λRS45 |
lacZ |
[35] |
|
|
λPC25 |
λRS45 |
dmsA-lacZ lacY+lacA+ |
[4] |
|
|
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Bearson et al. BMC Microbiology 2002 2:13 doi:10.1186/1471-2180-2-13 |
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