Figure 5.

Comparison of the NarL-phosphate protection patterns for the entire dmsA promoter region versus a truncated dmsA promoter fragment. The open box denotes the DNase I protected region for the entire dmsA region (Fragment A, -127 to +62) whereas the closed box indicates the protected region for the truncated dmsA promoter region (Fragment B, -127 to -13). Numbering of the DNA is relative to the start of dmsA transcription. Lane G contains the Maxam-Gilbert sequencing reaction. The noncoding strand of DNA was used in both fragments, and the NarL protein used in Lanes 3–5 and 7–9 was phosphorylated with acetyl phosphate prior to incubation with the dmsA fragment. The amount of NarL used in each lane is indicated above each lane.