Open Access Research article

Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan

Atsushi Hinenoya1, Kensuke Shima15, Masahiro Asakura1, Kazuhiko Nishimura1, Teizo Tsukamoto1, Tadasuke Ooka2, Tetsuya Hayashi2, Thandavarayan Ramamurthy3, Shah M Faruque4 and Shinji Yamasaki1*

Author Affiliations

1 Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku ourai-kita, Izumisano, Osaka 598-8531, Japan

2 Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan

3 National Institute of Cholera and Enteric Diseases, Kolkata 700010, India

4 Centre for Food and Water Borne Diseases, International Centre for Diarrhoeal Diseases Research, Bangladesh, Dhaka 1212, Bangladesh

5 Current address: Institute of Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

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BMC Microbiology 2014, 14:97  doi:10.1186/1471-2180-14-97

Published: 18 April 2014

Abstract

Background

Cytolethal distending toxin (CDT)-producing Escherichia coli (CTEC) has been isolated from patients with gastrointestinal or urinary tract infection, and sepsis. However, the source of human infection remains unknown. In this study, we attempted to detect and isolate CTEC strains from fecal specimens of healthy farm animals and characterized them phenotypically and genotypically.

Results

By PCR analysis, the cdtB gene was detected in 90 and 14 out of 102 and 45 stool specimens of healthy cattle and swine, respectively, and none from 45 chicken samples. Subtypes of the cdtB genes (I to V) were further examined by restriction fragment length polymorphism analysis of the amplicons and by type-specific PCRs for the cdt-III and cdt-V genes. Of the 90 cdtB gene-positive cattle samples, 2 cdt-I, 25 cdt-III, 1 cdt-IV, 52 cdt-V and 1 both cdt-III and cdt-V gene-positive strains were isolated while 1 cdt-II and 6 cdt-V gene-positive were isolated from 14 cdtB positive swine samples. Serotypes of some isolates were identical to those of human isolates. Interestingly, a cdt-II gene-positive strain isolated from swine was for the first time identified as Escherichia albertii. Phylogenetic analysis grouped 87 E. coli strains into 77 phylogroup B1, 6 B2, and 4 D, respectively. Most of the B1 strains harbored both lpfAO113 and ehaA. Three and twenty-two cdt-V gene-positive strains harbored eaeA and stx genes, respectively, and seven possessed cdt-V, stx and subAB genes. The cnf2 gene, normally present in cdt-III gene-positive strains, was also detected in cdt-V gene-positive strains.

Conclusions

Our results suggest that healthy cattle and swine could be the reservoir of CTEC, and they could be a potential source of human infections.

Keywords:
Escherichia coli; Cytolethal distending toxin; Escherichia albertii; Molecular typing