Table 2

Bacterial strains and plasmids used in this study
Strain Description Reference or source
Pseudomonas syringae
pv. glycinea PG4180 Wild type, levan+ R. Mitchell
pv. phaseolicola 1448A Wild type, levan+ [33]
pv. syringae B728a Wild type, levan+ [34]
pv. tomato DC3000 Wild type, levan+ D. Cuppels
Pseudomonas syringae pv. glycinea PG4180
PG4180.M6 Spr, Gmr, lscB lscC mutant of PG4180, levan- [10]
PG4180.M6(pRA3.1) Spr, Gmr, Tcr, lscB lscC mutant of PG4180, containing lscA under control of Plac on 3.1-kb PstI fragment in pRK415 [10]
Escherichia coli
DH5α supE44 DlacU169 (F80 lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [31]
Plasmids
pRK2013 Kmr, helper plasmid [35]
pLB7.2 Apr, contains lscB on 7.2-kb EcoRV insert [10]
pBBR1MCS Cmr, broad-host-range cloning vector [36]
pBBR1MCS-3 Tcr, broad-host-range cloning vector [36]
pBBR3-500-lscB Tcr, lscB gene with −500-bp upstream sequence in pBBR1MCS-3 [24]
pBBR3(lscA) Tcr, lscA gene containing insert from pRA3.1 in PBBR1MCS-3 not under control of Plac This study
pBBR3(lscBUpNA) Tcr, fusion of 518-bp upstream region of lscB (including first 48-bp of coding region) and lscA (including start codon and downstream region) in pBBR1MCS-3 This study
pBBR3(lscBUpA) Tcr, fusion construct of 470-bp upstream region of lscB (without N-terminus) and lscA (including start codon and downstream in pBBR1MCS-3 This study
pBBR3(lscAUpB) Tcr, fusion of 550-bp upstream region of lscA and lscB (including start codon and downstream region) in pBBR1MCS-3 This study

Ap, Ampicillin; Cm, Chloramphenicol; Gm, Gentamycin; Km, Kanamycin; Sp, Spectinomycin; Tc, Tetracycline; r, resistant.

Khandekar et al.

Khandekar et al. BMC Microbiology 2014 14:79   doi:10.1186/1471-2180-14-79

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