Expression of lscA in different P. syringae pathovars. The bacterial cells were harvested at OD600 of 0.5 and 2.0. Total RNA was extracted as described in the Materials and Methods followed by generation of cDNA. PCR amplification of lscA fragment on the total cDNA using strain-specific primers showed no amplicon (lscA panel) indicating no expression of lscA. Quality of the primers was checked by performing PCR amplification using genomic DNA (gDNA) as template. Amplification using an unrelated gene hexR (hexR) and artificially expressed lscA by Plac [M6(pRA3.1)] signified correct reverse transcription.
Khandekar et al. BMC Microbiology 2014 14:79 doi:10.1186/1471-2180-14-79