Additional file 4: Figure S4..

(A) IL-12 production in the ascites fluid of infected mice. Wild type mice were infected intraperitoneally with T. gondii tachyzoites. At 3 and 5 days post-infection (dpi), IL-12 production in the ascites fluid was measured. Each value represents the mean ± the standard deviation of four replicate samples. A significant increase in IL-12 production was seen in the mice infected with RH-OE at 3 dpi compared with those infected with RH-GFP or RH-DN. (B) Recruitment of immune cells. Wild type mice were infected intraperitoneally with T. gondii tachyzoites. At 3 days post-infection (dpi), peritoneal cells were harvested from uninfected or parasite-infected mice. Cells were then subjected to flow cytometry to determine the absolute number of cells expressing CCR5, CD11b, CD11c, or CD3. Each value represents the mean ± the standard deviation of four replicate samples. RH-OE infection enhanced the recruitment of CD11b+, CCR5+, and CD3+ cells compared with RH-GFP or RH-DN infections.

Format: TIFF Size: 645KB Download file

Ibrahim et al. BMC Microbiology 2014 14:76   doi:10.1186/1471-2180-14-76