Characterization of recombinant parasites. (A) Western blot analysis of T. gondii tachyzoites of RH-WT and RH-OE clones (C1, C2 and C3). (B) Secretion of TgCyp18 from extracellular parasites of RH-OE clones at 30 min incubation. Each value represents the mean ± the standard deviation of triplicate samples. (C) Secretion of TgCyp18 from RH-WT, RH-GFP and RH-OE (clone C2) extracellular parasites. Each value represents the mean ± the standard deviation of triplicate samples. (D) TgCyp18 secretion in the ascetic fluid of infected mice at 3 and 5 days post-infection (dpi). Tachyzoites were inoculated intraperitoneally into wild type mice. Each value represents the mean ± the standard deviation of four replicate samples. Results are representative of two repeated experiments with similar results. RH-WT: wild-type parasites; RH-GFP: parasites transfected with GFP; RH-OE: parasites transfected with TgCyp18HA and GFP.
Ibrahim et al. BMC Microbiology 2014 14:76 doi:10.1186/1471-2180-14-76