In vitro interaction between RbaW and RbaV. Pull-down assays were done using NHS bead-conjugated recombinant RbaW supplemented with recombinant RbaV or control protein (lysozyme). Conjugated control beads (Lanes 1 and 2) were not supplemented with test protein while non-conjugated bead controls (Lanes 3 and 6) were blocked by 100 mM Tris. Both N- and C-terminal 6x-His-tagged RbaW proteins were conjugated and tested against N-terminal 6x-His-tagged RbaV (Lanes 4 and 5, respectively). Lysozyme (14.3 kDa) was tested against RbaW-conjugated beads (Lanes 7 and 8) as a control. The gel was stained with Coomassie blue and the resulting image was adjusted for brightness and contrast. Molecular weight references are indicated on the left of the gel.
Mercer and Lang BMC Microbiology 2014 14:71 doi:10.1186/1471-2180-14-71